11 Immunodiagnostics Flashcards
poor sensitivity results in
false negatives - didnt detect it when it was there
poor specificity results in
false positives - detected something when it wasnt there or something else made your test pop hot
precipitin reaction positive
forms a complex that visibly precipitates
agglutination reaction positive
cells/particles bound together through Ab-Ag rxn
Labeled Ab methods
Ab linked to label
Ab bound to Ag detected by presence of label
direct tests detect
Antigen
indirect tests detect
antibodies
direct flourescent Ab test will ID what
Antigen in tissue - flourescent Ab tags against Ag interact and are detected by the light
indirect flourescent Ab test will ID waht
Ab in patient - flourescent molecule that binds Ab added - if patient Ab is attached to Ag testing for it will bind flourescent molecule and get detected
precipitation will only occur with
multivalent Ag and Ab crosslinking to form large aggregates
ouchterlony double diffusion test can determine what and with what visible rxn
can determine if Ag contain overlapping epitopes
place Ab in the middle of different Ag- if precipitation occurs, it reacts with that Ag
can determine if they are identical, different, if there is crossreaction
agglutination test is typically used in
blood typing
direct coombs test
detects anti-Rh Ab on fetal RBCs
indirect coombs test
detects anti Rh Ab in mothers serum
