11. Clinical Applications of FISH/real-time PCR Flashcards by E E | Brainscape

Q

For detection of translocation when chromosome partners are KNOWN

A

Fusion FISH probe

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Q

Drawback of break-apart FISH probe?

A

Cannot determine translocation partner with this method - only the PRESENCE of a translocation

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Q

For detection of translocation when chromosome partners are UNKNOWN

A

Break-apart FISH probe

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Q

Both probes contribute to the signal when a translocation has been detected. Which type of probe is this?

A

Fusion FISH probe

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Q

Assay with the most diagnostic sensitivity for detection of a previously undetermined t(14,18) translocation

A

FISH

Picks up more translocations if breakpoints are KNOWN

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