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MOLECULAR BIOLOGY > 1 - LABORATORY > Flashcards

1 - LABORATORY Flashcards

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1
Q

Specialized in DNA cloning and recombinant DNA technology

A

Cloning lab

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2
Q

Handle different cell lines
Diff type of cells
Diff cell assays

A

Cell culture laboratory

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3
Q

basta RNA

A

RNA lab

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4
Q

Study makes use of yeasts as a model organism (laboratory)

A

Yeast laboratory

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5
Q

Bacteria processing experiments

A

Bacteria laboratory

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6
Q

Large animals, basta animals experiments

A

Animal laboratory

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7
Q

Limits what you can and cannot do in a laboratory

States which organisms or viruses could be handled

Take into account the safety of the personnel

It changes the rules and equipment and samples in the laboratory

A

Biosafety level

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8
Q

General laboratory biosafety level

A

BSL 1

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9
Q

Handles infectious microbes biosafety level

A

BSL 2

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10
Q

Higher level of containment biosafety level

A

BSL 3 and 4

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11
Q

The neatest type of laboratory in any institute

For general use of everyone

A

Bates College General Equipment Laboratory

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12
Q

Largest general laboratory for molecular biology

A

National Institute of Molecular Biology and Biotechnology - UP DILIMAN

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13
Q

Ewan basta lab siya example na may sink daw

A

Dougherty Laboratory at Caltech

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14
Q

Has reverse microscope

A

University of Copenhagen - GLOBE Institute

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15
Q

Tip boxes daw

A

Molecular Immunology Laboratory at Sabanci Universitesi

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16
Q

Universal precaution

A

All specimens treated as pathologic

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17
Q

Should have plastic caps and contains reagents in different volumes

Should have label

A

Reagent bottles

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18
Q

Mixing reagents equipment

A

Erlenmeyer flask and beakers

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19
Q

Measurement of larger volumes

A

Graduated cylinders

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20
Q

Used for corrosive agents for stocked solutions pipet

A

Glass pipettes

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21
Q

Pipet used in serology used to transfer small volumes

A

Serological pipettes

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22
Q

Used in cell culture

A

Pasteur Pipette/Plug Pasteur Pipette

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23
Q

of different sizes

used for containing material to be centrifuged

A

Centrifuge tube

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24
Q

Smaller centrifuge tubes

A

Microcentrifuge tubes

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25
Q

instead of using small tubes one by
one, we have this strip so you won’t lose all.

Be careful when taking off the cap.

A

Tube strip for PCR

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26
Q

it can be a six wall plate, twelve
wall plate, etc.

A

Plates for different assays

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27
Q

this is what you put on a micropipette or
pipettor

A

Pipette tips

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28
Q

used for PCR (esp. for Quantitative PCR)
since we deal a lot of samples for that kind of experiment.
Sometimes you would consume all 96 tubes in one run

A

Tube Rack

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29
Q

for different uses not only for culturing as it can also be used for staining

A

Dishes

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30
Q

-80°C freezer

used for Pfizer vaccine

A

Ultra low freezer

31
Q

Prize of ultra low freezer

A

700k or more

32
Q

Preferred basic refrigerator

A

Two door refrigerator

33
Q

depends on enzyme using,
what temperature do they work best

A

Reaction Temperatures

34
Q

for cultures, what temperature do they grow (usually at 37°C, but sometimes at 40°C or above or sometimes lower depending on the experiment)

A

Incubation Temperatures

35
Q

depending on where you are, the temperature can range from 24°C or if from the Philippines, it can be from 35°C, 36°C, 37°C

A

Ambient/RT (Room Temp)

36
Q

Where to store certain materials requiring certain temperatures

A

4°C (Refrigerator temperature),
-20°C (Freezer temperature(refrigerator))
-60°C (Different kind of refrigerator)
-80°C (Ultra low freezer), in liquid nitrogen

37
Q

Pipette useful for cell culture

A

Electronic pipette

38
Q

1,000 microliter color of tip pipettor

A

Blue tip

39
Q

200 microliter color of tip pipettor

A

yellow tip

40
Q

10 microliter or smaller color of tip pipettor

A

white tip

41
Q

Pipettor that has multiple tips

A

Multichannel pipettor

42
Q

Multichannel pipettor is important in what types of experiments

A

Enzyme Linked Immunosorbent Assay

43
Q

Used for measuring

weights of reagents
hydroscopic reagents
analytical balance
pH Meter

A

Top loading balance

44
Q

Important in dealing with reagents that are effervescent, if they produce fumes

A

Fume hood

45
Q

Workspace especially when doing cultures of microbes

Class 2 cabinet which means that the filtered air leaving it contains no pathogen

Top part of such has filter system preventing any particulates, any pathogens from leaving

A

Biosafety cabinet

46
Q

Creates class 1 and class 2 water depending on what you need

It can distil or ionize it depending on the
experiment you need it for

A

Filtration system

47
Q

+ Can be any storage container
+ This is where we store the water that has been
treated

A

Storage Container Jack

48
Q

Biosafety cabinets uses what filters

A

HEPA filter

high efficiency particulate air

49
Q

Sterilizing equipment

A

Standing autoclave
Drying oven

50
Q

Sterilizing by wet heat

A

Standing autoclaveS

51
Q

Standing autoclaves are giant pressure cookers that sterilize materials by wet heat at _______ temperature and ____________ pressure for ____________ minutes

A

121 degrees Celsius
15 pounds per square inch
15-20 minutes

52
Q

Sterilization by dry heat

A

Drying oven

53
Q

Drying oven temp

A

300 degrees Celsius for a long period of time

54
Q

Think of it as high tech flame test

Measure the amount of ions that you have depending on the flame produced

Very complicated and expensive machine

A

Atomic Absorption Spectrophotometer

55
Q

use a light source to illuminate a sample with light across the UV to the visible wavelength range (typically 190 to 900 nm).

The instruments then measure the light absorbed, transmitted, or reflected by the sample at each wavelength

A

UV-visible (UV-Vis) spectrophotometers

56
Q

Incubators

A

Shaking Microbial Incubator
Cell Culture Incubator

57
Q

Any laboratory needs this especially if doing cloning work since a lot of the cloning work use E. coli. Whether grown on a plate or liquid
medium, needs an incubator to grow

A

Microbial Incubator

58
Q

for cell culture if growing eukaryotic cells needs them to be in condition wherein there is a continuous carbon dioxide in the atmosphere

A

Carbon Dioxide Incubator

59
Q

For microcentrifuge tubes
For spinning down reagents

A

Tabletop centrifuge

60
Q

For conical tubes

A

Refrigerated centrifuge

61
Q

Used for washing blots, gels, membranes and different staining specimens

A

Orbital Shaker

62
Q

Some reagents require heating and stirring for a very long time, that is why we use this machine

A

Hot Plate with Stirrer

63
Q

Such little knob at the top vibrates at a high speed so that when you put a microcentrifuge tube beside it, vibrations agitates the contents of the tube and the mixes it

A

Vortex mixer

64
Q

For tissue homogenization

Releases high frequency sound waves in order to create vibrations

A

Sonicator Setup

65
Q

Used to dissolve agarose in the buffer and used to make the gel. Important in any electrophoresis laboratory

A

Microwave

66
Q

Apparatus for Agarose Gel Electrophoresis

A

Tap
Power Supply
Protein Electrophoresis Unit
Imaging System

67
Q

In Agarose Gel Electrophoresis, this is where the gel is placed

A

Tap

68
Q

Such creates the cathode and anode and that DNA molecule would move towards the postive

A

Power Supply

69
Q

Two parts of the Protein Electrophoresis Unit

A

Vertical Unit - samples go down
Power Supply

70
Q

Either gel or blot, it is able to capture pictures

A

Imaging system

71
Q

Used for transferring DNA or RNA in a gel

A

Transfer

72
Q

If there’s a gel being stained, simply put it here to dry

A

Drying apparatus

73
Q

a device used to test and study the degradation of materials which are subjected to continuous cycling between hot and cold temperatures during their service life.

A

Thermocycler

MOLECULAR BIOLOGY (7 decks)

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