1. Introduction Flashcards
Length og DNA
2m/nucleus
Difference between human and chimpanzee genomes
Only 4%
Homolog
DNA sequence from common ancestral gene
Ortholog
Genes in different species that evolved from a common ancestral gene by speciation. Normally, orthologs retain the same function in the course of evolution
Paralog
Generated by duplication within the same genome, may gain new functions
How similar is the genome of two individuals?
99.5% identity
Definition of CpG islands
- Regions of DNA of at least 200 bp in length
- G+C content >50%
- A ratio of observed vs. expected CpGs close to or >0.6
- (Cytosine, phosphodiester bond, guanine)
CpG islands found in or near approximately 40% of….. of mammalian genes
Promoters
Amount of DNA in somatic cells differ based on..?
- Presence and number of nuclei
- Different stages of cell cycle
Is the DNA sequence identical in all somatic cells?
No, in T cells and B cells it is different (somatic gene rearrangement)
DNA encodes for
- RNA and proteins
- Lipids and carbohydrates are non-templated
Genome
The complete genetic material of the cell
Genetics
Science focusing on individual genes, the inheritance and variability
Genomics
Science focusing on the entire genome and the interaction of genes
Epigenetics
Science focusing on inherited, however not DNA sequence encoded changes of gene expression
(The study of changes in organisms caused by modification of gene expression rather than alteration of the genetic code itself)
The active strand that participates in transcription of mRNA
Antisense or non-coding strand. Transcribes the sense mRNA
The nucleosome consist of
8 histone molecules + 146 nucleotide pairs of DNA