1. DNA Flashcards

1
Q

What are the 3 components that make up a nucleotide?

A
  • pentose sugar
  • phosphate group
  • nitrogenous base
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2
Q

DNA acts as a template to make RNA in a process known as ______?

A

Transcription

* which takes place in the nucleus

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3
Q

RNA acts as a template to make protein in a process known as ______?

A

Translation

* which happens in the cytoplasm on ribosomes

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4
Q

Purines are made up of ___ ring(s)

A

2

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5
Q

Pyrimidines are made up of ____ ring(s)

A

1

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6
Q

A forms ___ H bonds with T

C forms ___ H bonds with G

A

A forms 2 bonds with T

C forms 3 bonds with G

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7
Q

Purines :

Pyrimidines:

A

A and G are purines

C and T are pyrimidines

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8
Q

Where do phosphodiester bonds form ?

A

Between the pentose sugar and the phosphate group

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9
Q

Why don’t RNA have H bonds?

A

Because single-stranded. Only have phosphodiester in sugar-phosphate backbone. No need to hold 2 nitrogenous bases tgt

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10
Q

How to break down polynucleotides

A

Hydrolysis (+ water)

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11
Q

How to form polynucleotides from nucleotides?

A

Condensation polymerisation/ dehydration synthesis (- water)

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12
Q

Function of helicase

A

Convert double stranded DNA into single-stranded DNA by breaking hydrogen bonds

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13
Q

Function of single-stranded binding protein

A

To bind on single strands of DNA and prevent them from reannealing

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14
Q

Function of primase

A

To create RNA primers based on template strand

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15
Q

Function of DNA polymerase III

A

To add complimentary DNA nucleotides to the 3’ end, based on the template

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16
Q

Function of sliding clamp

A

To move DNA polymerase III along the template strand

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17
Q

Function of nuclease

A

To remove RNA primers

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18
Q

Function of DNA polymerase I

A

To replace RNA nucleotides with DNA nucleotides

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19
Q

Function of DNA ligase

A

To join the replicated DNA fragments into a single continuous strand

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20
Q

Other types of monohybrid inheritance

A
  1. Incomplete dominance
  2. Co dominance
  3. Multiple alleles
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21
Q

What do multiple alleles mean?

A

When one gene has more than two alleles

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22
Q

3 exceptions to Mendels law

A
  1. Polygenic inheritance
  2. Linked genes
  3. Effect of environment
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23
Q

What happens before Prophase?

A

Interphase

- G1, S & G2

24
Q

What happens after Prophase?

25
What is the order of mitosis?
``` Interphase Prophase Metaphase Anaphase Telophase ``` Cytokinesis (not a part of mitosis)
26
What happens during Prophase?
- Chromosomes have been duplicated - Centrioles have been duplicated - Central start producing spindle fibers - Nuclear envelope starts to break down
27
What happens during Metaphase?
Duplicated chromosomes are lined up at the Metaphase plate/ equator
28
What happens during Anaphase?
- Sister chromatids are separated/ Duplicated chromosomes are split at the centromere (spindle fibers pull the chromatids to opposite poles) - Nuclear envelope still not present - Cleavage furrow starts to form
29
What happens during telophase?
- Unreplicated chromosomes reach opposite poles - Spindle fibers breakdown - Nuclear envelope starts to form again - Cleavage furrow forms
30
Cytokinesis
- Cytoplasm is divided - Two nuclei are separated - Two new daughter cells are formed
31
Two functions of mitosis
Asexual reproduction Growth and repair
32
When may crossing over occur?
During prophase I
33
When does independent assortment occur?
During metaphase I
34
What happens in Anaphase I
Homologous chromosomes separated
35
What happens in Anaphase II
Sister chromatids separated
36
Genotype
The genetic makeup of an individual (Aa / AA / aa)
37
True breed
A.k.a. homozygous | Two copies of the same allele
38
Hybrid
A.k.a. heterozygous | Two different copies of an allele (Aa)
39
What are the three laws in inheritance
Law of dominance Law of segregation Law of independent assortment
40
Law of independent assortment
The segregation of one pair of alleles is independent of another pair of alleles One pair of alleles is segregated independently of another pair of alleles
41
Law of segregation
When gametes are produced, alleles are segregated
42
AA x aa
100% Aa | Heterozygotes
43
Aa x Aa
1 AA : 2 Aa : 1 aa 3 dom phe: 1 rec phe
44
Aa x aa
1 Aa : 1 aa | 1 dom phe : 1 rec phe
45
AaBb x aabb
1AaBb : 1 Aabb: 1 aaBb : 1 aabb
46
If we don’t get 9:3:3:1 ratio..?
1. Chance | 2. Linked genes
47
Hemizygous
Only have one copy of an allele
48
X linked genes | Y Linked genes
Genes located on X or Y chromosome
49
Autosomal dominant
When both parents have the trait but produce offspring that don’t have the trait
50
Autosomal recessive
When both parents don’t have the trait but produce offspring that have the trait
51
Y linked Inheritance
Only males affected | Affected fathers produce affected sons
52
X-linked recessive
More affected males than affected females Affected fathers WONT produce affected sons Affected mothers produce affected sons
53
X linked dominant
More affected females then affected males Affected fathers produce affected daughters
54
Transcription stages
Initiation Elongation Termination
55
Polygenic inheritance
When a character is controlled by more than one gene With multiple genes work together to produce a single character * Continuous variation
56
Dihybrid x dihybrid | AaBb x AaBb
Phenotypic ratio: | 9:3:3:1
57
Linked genes
Located on the same chromosome close to each other