1. Cell Structure Flashcards
A millimetre is 1x10^?
1x10^ -3
A micrometre is 1x10^?
1x10^ -6
A nanometre is 1x10^?
1x10^ -9
What is an eyepiece graticule?
a small, transparent scale of 100 divisions that can be placed in a microscope eyepiece to measure cells and organelles
What is a stage micrometer?
a mini, transparent ruler engraved on a microscope slide that can be placed on the microscope stage to calibrate the eyepiece graticule
What is resolution?
the ability to distinguish between 2 separate objects that are very close together
Higher resolution?
greater DETAIL
What is the maximum resolution and magnification of a light microscope?
res.: 200nm
mag.: x1500
What is the maximum resolution of an electron microscope?
res.: 0.5nm
mag.: x500,000
What is the difference between transmission and scanning electron microscopes?
transmission: produces 2D images
scanning: produces 3D images
2 advantages of electron microscopes
1) metal stains can be added to highlight key structures
2) the images always come out on computer, so false colour can be added
5 disadvantages of electron microscopes
1) specimens must be dehydrated, so only dead specimens can be examined
2) requires a machine (ultramicrotome) that slices the specimen to ensure its really thin; time consuming and not cost effective
3) dull blades on the ultramicrotome can disturb organelles and create artefacts
4) expensive
5) immobile
What are prokaryotes and eukaryotes?
prokaryote:
- lacks nucleus and other organelles
- unicellular
- includes bacteria
eukaryote:
- multi-celled organism
- animal and plant cells
Draw and label an animal cell (16 labels)
nucleus (nucleolus, nuclear envelope, chromatin), cytoplasm, cell membrane, lysosome, mitochondria, 80s ribosomes, 70s ribosomes in the mitochondria, centriole, golgi apparatus, RER and SER, vesicles, microvilli
What is the function of the nucleus?
contains genetic material and controls cell activities
What is the function of the nucleolus?
manufactures ribosomes, which are made up of ribosomal proteins and rRNA (ribosomal RNA)
What is the nuclear envelope?
2 lipid bilayer membranes that provide structure and separate the contents of the nucleus from the cytoplasm
What is the function of chromatin?
consists DNA, proteins, and RNA
used to make chromosomes