06 - bone marrow evaluation

Question 1

(sites for collection)

1. dogs?
2. cats?

Answer 1

1. proximal femur/humerus, iliac crest
2. proximal femur/humerus

Question 2

(indications)

1. 5 of them

Answer 2

1. non-regen anemia, neutropenia, thrombocytopenia, gammopathies, possible neoplastic bone marrow disease

Question 3

(erythroid cells)

1. what is the maturation sequence?

Answer 3

rubriblast -> prorubricyte -> rubricyte -> metarubricyte -> polychromatophilic eryhtrocyte -> mature RBC

Question 4

1. what happens from rubriblast to RBC?

Answer 4

1. lose nucleus, become smaller, ^ cytoplasm, lose ability to undergo mitosis

Question 5

(granulocyte (myeloid) cells)

1. what is the maturation sequence?
2. what happens as they mature?
3. what 3 things do they become?
4. what happens to nuclei as they go to final form?

Answer 5

1. myeloblast -> promyelocyte -> myelocyte -> metamyelocyte -> band neutrophil -> segmented neutrophil
2. ^ granules, become smaller, lose mitosis ability,
3. neutrophils, eosinophils, basophils
4. change from kidney bean to band

Question 6

(monocyte cells)

1. monoblasts -> promonocytes -> monocytes
2. difficult to distinguish from myeloid series

Question 7

1. megakaryocytes make what?
2. maturation sequence?

Answer 7

1. platelets
2. megakaryoblasts -> promegakaryocytes -> megakaryocytes

Question 8

other cells in bone marrow

Question 9

(interpretation)

1. normal cell/fat ratio?
2. how many megakaryocytes per slide?
3. normal myeloid:erythroid (M:E) ratio?

↓ suggests what?

^ suggests what?

Answer 9

1. 1:1
2. 5-10
3. 0.5-3:1

^ RBC production, ↓ neutro production, or both

the opposite…

Question 10

(interpretation)

1. what % of the cells should be more mature?
2. disorderly maturation suggests what?

Answer 10

1. 80 to 90%
2. leukemia, myeloid hyperplasia, IMHA, marked inflammation