05 Learning about DNA, RNA and Protein through CRISPR-Cas9 Flashcards
1
Q
CRISPR stands for
A
Clustered regularly interspaced short palindromic repeats
2
Q
Where is CRISPR from?
A
Prokaryotes (bacteria) immune system against viruses / bacteriophages
3
Q
Types of short RNA in CRISPR
A
guide RNA (variable, complementary to viral DNA)
tracr RNA (constant, scaffolds with Cas9 protein)
fuse both - sgRNA (single guided RNA)
4
Q
What is PAM?
A
Protospacer adjacent motif
2-6 base sequence of DNA immediately following the DNA sequence targeted by Cas9 -> avoid targeting own (self) DNA
5
Q
What is dCas9?
A
Dead Cas9 - engineered to have binding ability but cannot cut DNA
6
Q
Uses of CRISPR today
A
Gene editing in labs / human cell lines / organoids
Non-medical applications - crops, biofuels
