Zebrafish

Question 1

Which of the three components of the fertilised zebrafish embryo – the yolk, the chorion, the cell – contains the genetic material?

Answer 1

The cell

Question 2

What event happens at mid-blastula transition?

Answer 2

This is the stage at which the embryo starts transcribing its own genome. Up to then, the embryo was only using the mRNAs deposited in the egg by the mother prior to fertilization.

Question 3

What is the name of the three layers formed during gastrulation?

Answer 3

ectoderm, mesoderm, endoderm.

Question 4

What are the developmental stages ?

Answer 4

1. Zygote
2. Cleavage
3. Blastula
4. Gastrula
5. Segmentation
6. Organogenesis (pharyngula, hatching, larval )

Question 5

When does the heart start beating in a zebrafish embryo?

Answer 5

The heart starts beating at around 36 to 48 hours post-fertilisation (hpf). Sometimes you can already see it beating as early as 28 hpf!

Question 6

When does the embryo start moving?

Answer 6

Before coming out of the chorion

Question 7

Structure of developed zebra fish

Answer 7

Hatching gland 
Eye
Forebrain 
Midbrain
Hindbrain
Ear
Spinal chord
Notochord 
Somites 
Fin fold 
Blood island 
Yolk