Yields in culture Flashcards
What can yield principles be used for?
Can be applied to cell metabolism to relate the flow of substrate in metabolic pathways to the formation of biomass and other products.
What do yield coefficients allow us to do?
Allow us to quantify the nutrient requirements and production characteristics of organisms.
Give the generalised definition of a yield coefficient
Give the symbols and definitions of some metabolic yield coeffiencts
What is the problem of the yield coefficient equation?
What are the implications of this?
Values of ΔJ and ΔK depend on the time period over which they are measured.
ΔJ and ΔK can be calculated as the difference between initial and final states; this gives an overall yield representing an average value over the entire culture period. Alternatively, ΔJ and ΔK can be determined between two other points in time; this calculation might produce a different result for YJK
As yields can vary during culture, we may have to calculate the instantaneous yield of a batch. This process is seen in the figure:
What is the observed biomass yield and how does it compare to theoretical yield?
In reality, is all the substrate that is consumed used for cell growth?
No, If the total mass of substrate consumed is St, some proportion of St equal to Sg will be used for growth while the remainder, Sr, is channelled into other products and metabolic activities not related to growth
