Yeast And Wine Flashcards
Yeasts have two different Metabolisms
Fermentative Metabolism –> Ethanol –> 2 ATP
2. Respiratory Metabolism –> Biomass (no ethanol) –> 36 ATP
ATP
The principal molecule for storing and transferring energy in cells.
Respiration and glycosis
During the glycolysis, glucose is broken down to produce 2 molecules of 3 carbons, the pyruvate.
YEAST REHYDRATION & ACCLIMATION
During the phases of rehydration and acclimation, the respiratory metabolism should be pushed by increasing the amount of dissolved oxygen (DOT) and avoiding high concentrations of sugar, which would stimulate fermentation due to the Crabtree effect.
Acclimation
- The cell senses the environment via protein molecules on its surface, i.e. the cell membrane.
- A proper acclimation procedure allows a stepwise contact between the yeast population and a new environment, grape must in our case. This procedure stimulates the growth even in the early phases of fermentation, thus reducing the length of the lag- phase.
What happens with no oxygen during ferm
If however no oxygen is present in the environment, yeast degrade pyruvate by converting it into ethanol.
Crabtree Posititve
Certain yeasts possess the ability to produce ethanol even in presence of oxygen !!!
Yeasts demonstrating this phenomenon are characterised as Crabtree-positive.
Crabtree positive yeast (3)
Saccharomyces cerevisiae
1. Ferment sugar in addition to respiration
2. In presence of oxygen
3. Above a critical [sugar]
Crabtree negative
However, not every yeast exhibit this phenomenon. Therefore, yeast consuming sugar only under the respiration pathway are characterised as Crabtree-negative.
Also they use solely the respiration pathway
What kind of yeasts can we use in Wine Making
Crab-tree Positive yeasts
Four phases of Yeast
- Lag Phase
- Exponential Phase
- Stationary Phase
- Death Phase
Lag phase
Respiration doesnt stop completely
Exponential Phase
Respiration stops
Stationary Phase
To control the number of
living cells, methilin blue can be used
to color dead cells.
Death phase
If too many cells die, fermentation will stop