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Microbiology Rotation > Wound Bench > Flashcards

Wound Bench Flashcards

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1
Q

Ideal way to collect a specimen from a wound

A

Asperate

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2
Q

Ideal way to transport a wound specimen to the lab

A

As soon as possible in specific transport media

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3
Q

Delay shorter than 6 hours

A

kept at ambient temperature

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4
Q

Delay longer than 6 hours

A

refrigerated

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5
Q

What is specimen collection is preferred?

A

Specimens aspirated with a needle and syringe are preferred to those collected on swabs

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6
Q

Common submitted specimen

A
  1. Sterile body fluids
  2. Biopsy/Tissue
  3. Eye
  4. Ear
  5. Foreign body
  6. Catheter tips
  7. Wounds
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7
Q

What media should be inoculated from a wound?

A
  1. BAP
  2. CHOC
  3. MAC
  4. CNA
  5. ANA BAP
  6. PEA
  7. THIO
  8. Direct Smear
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8
Q

Purpose of BAP

A

All purpose medium, best for hemolysis, detects stalemating of Haemophilus around organisms such as Staphylococcus aureus

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9
Q

Purpose of MAC

A

Selective for Gram negative rods, (look for Lactose pink). (does not determine fermenter or non fermenter of glucose)

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10
Q

Purpose of CNA

A

Selective for Gram positive organism (do not determine hemolysis)

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11
Q

Purpose of CHOC

A

Enriched medium that will grow most any organism (supports fastidious organisms)

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12
Q

Purpose of ANA BAP

A

Prereduced, growth supplements added (enriched with Vitamin K. Hemin and cysteine)

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13
Q

Purpose of PEA

A

Phenyl Ethyl Alcohol Agar

Inhibits swarming of proteus

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14
Q

Purpose of THIO

A

Enhances growth of aerobes, facultative organisms, as well as obligate anaerobes

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15
Q

Purpose of direct smear

A

Very important to correlate with plate morphology

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16
Q

How does gas gangrene (myonecrosis) present on a gram stained direct smear from an infected wound

A

Necrotic background and gas bubbles

17
Q

How long are anaerobic plates typically incubated before initial examinations?

A

2 days (48 hours)

18
Q

How is quantitation determined for wound cultures

A

Quantitation is dependent on the quadrant where organisms are growing (1+ first quadrant; 2+ second quad, 3+ third quad, 4+ fourth quadrant)

19
Q

What skin flora organisms would likely be seen in surface wound cultures

A
  • Alpha and Gamma Strep
  • Corynebacterium spp
  • Coag Neg staph
  • Propionibacterium spp.
  • Staphylococcus saccharyolyticus
  • staphylococcus epidermidis
20
Q

Biochemical Tests Used to Identify:

- Coag Neg Staph

A

Gram Stain: GPC in clusters

Coagulase test: neg

21
Q

Biochemical Tests Used to Identify:

- Corynebacterium spp

A

Gram Stain: GPR palisading
Catalase: pos
Nonmotile
opaque and or alpha

22
Q

Biochemical Tests Used to Identify:

- Strep Viridian’s (alpha or gamma strep; not group D)

A

Gram Stain: GPC in chain

23
Q

Biochemical Tests Used to Identify:

- Propionibacterium spp.

A

Anaerobe

24
Q

Most common organism in burns

A

Pseudomonas aeruginosa
MRSA
Enterococcus
Klebsiella

25
Q

Conventional biochemical tests (and reactions) that would be used to identify the organisms that are in burns

A

Pseudomonas aeruginosa is Oxidase Positive

26
Q

Most common organism causing Impetigo, Erysipelas, Cullulitus, Folliculitis, Furuncle, Carbuncle, etc.

A

Staph aureus

27
Q

Conventional biochemical tests (and reactions) that would be used to identify the organisms that cause Impetigo, Erysipelas, Cullulitus, Folliculitis, Furuncle, Carbuncle, etc.

A

S. aureus:

  • Cat +
  • Coagulase +
  • Beta hemolytic
  • Furazolidone Susceptible
  • Novobiocin Susceptible
28
Q

Most common organism causing dog/cat bite infections

A

Pasteurella multocida/ canis, bacteroides spp., Fusobacterium, Prevotella, staph, capnocytopaga canimorsus

29
Q

Conventional biochemical tests (and reactions) that would be used to identify the organisms that cause dog/cat bite infections

A

GNCB

  • nonmotile
  • facultatively anaerobic
  • form nitrites from nitrates
  • ox pos
  • cat pos
30
Q

Most common organism causing human bite infection

A

Eikenella corrodens,

Straptococcus anginosis, S. aureus, Fusobacterium nucleatum, prevotella melaninogenica

31
Q

Conventional biochemical tests (and reactions) that would be used to identify the organisms that cause human bite infection

A 
E. corrodens
GNCB
nonmotile
ox pos
asaccharolytic 
cat neg
"pit" 
smell like chlorine
32
Q

Most common organism causing surgical wound (head neck) infection

A 
Mouth Flora anaerobes:  
Anaerobe: Prevotella spp. 
Alpha and gamma streptococci 
Corynebacerium spp
CoN Staph 
Non-Pathogenic Neisseria spp
Porphyromonas spp.
33
Q

Conventional biochemical tests (and reactions) that would be used to identify the organisms that cause surgical wound (head neck) infection

A

*

34
Q

Most common organism causing surgical abdominal wound infections

A 
GI flora: 
Bacteroides frag
Clostridium 
Bifidobacterium 
anaerobic cocci 
Fusobacterium 
E. coli (other Enterobacteriaceae) 
Enterocuccus
35
Q

Conventional biochemical tests (and reactions) that would be used to identify the organisms that cause surgical abdominal wound infections

A

*

36
Q

Most common organism causing Myonecrosis (gangrene)

A 
Clostridium perfringens (most common) 
- C. histolyticum, C. septicum, C. novyi
37
Q

Conventional biochemical tests (and reactions) that would be used to identify the organisms that cause Myonecrosis (gangrene)

A

GPR

  • double zone beta hemolysis
  • 4+ gas
38
Q

Most common organism causing Necrotizing fasciitis

A 
Enterobacteriaceae and anaerobes (type 1) 
S. pyogenes (type 2)
Clostridium perfringens (type 3 or Gas gangrene)
39
Q

Conventional biochemical tests (and reactions) that would be used to identify the organisms that cause necrotizing fasciitis

A

KIA series / anaerobe tests (GLC) / strep test

Microbiology Rotation (7 decks)

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