Miscellaneous Bench (CSF)

Question 1

Which CSF tubes should be sent to micro, hematology and chem lab?

Answer 1

Tube 1: Chemistry (contains skin plug thats ok)
Tube 2: Micro (most sterile)
Tube 3: Hematology (least amount of blood from traumatic stick)

Question 2

Direct smear:

- Haemophilus

Answer 2

pleomorphic gram negative rods so small you can’t tell if it is a GNR or cocci

Question 3

Direct smear:

- Neisseria

Answer 3

Gram Neg Diplococci (small)

Question 4

Direct smear:

- Listeria

Answer 4

Big enough to tell that it’s a rod

Question 5

Direct smear:

- E. coli

Answer 5

Medium to large gram variable rods

Question 6

Direct smear:

- Staph

Answer 6

*

Question 7

Direct smear:

- Strep

Answer 7

Gram positive cocci or football shaped if strep pneumo

Question 8

Common cause of meningitis in newborns

Answer 8

Group B strep
Listeria
e. coli

Question 9

Common cause of meningitis in young children

Answer 9

Haemophilus
Streppneumo
N. Meningitidis

Question 10

Common cause of meningitis in young adults

Answer 10

N. meningitidis

Question 11

Common cause of meningitis in order adults

Answer 11

S. pnemo
N. meningitidis
Listeria

Question 12

Common cause of meningitis in immunocompromised patients

Answer 12

Any and all

- fungi

Question 13

What condition would likely cause growth of anaerobes in CSF?

Answer 13

Brain abscesses

Question 14

How CSF should be stored during transportation or if they can’t be immediately processed?

Answer 14

CSF should NOT BE REFRIGERATED. RT or incubation

Question 15

CSF set up

Answer 15

If you have less 1ml you vortex and resuspend in the supernate; if you have >2 centrifuge for 20 mins.
CHOC, SBA, THIO, and two slides; Not enough fluid? Chocolate and slide