world of microscope Flashcards

1
Q

how do light microscopes work

A

they use a beam of light to form an image of the object

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2
Q

what magnification can light microscopes achieve

A

2000x

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3
Q

advantages of light microscopes

A

no radiation risk and are affordable

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4
Q

difference between light microscopes and electron microscopes

A

you can see live specimens on a light microscope but not an electron microscope as it uses a vacuum.

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5
Q

disadvantage of light microscope

A

low magnification and resolution ( make it difficult to see smaller objects)

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6
Q

importance of the development of electron microscopes

A

it enabled scientists to see and understand more about the subcellular structures in cells

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7
Q

How do electron microscopes work?

A

they use a beam of electrons to form an image

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8
Q

What magnification can electron microscopes achieve?

A

up to x2,000,000

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9
Q

What do transmission electron microscopes do?

A

they give 2D images with high magnification and resolution

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10
Q

What advantages are there to using electron microscopes?

A

higher magnification and resolution

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11
Q

What disadvantages are there to using electron microscopes?

A

they are large, very expensive and have to be kept in special temperature, pressure and humidity rooms

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12
Q

How do you calculate magnification with a light microscope?

A

multiply the eyepiece lens by the magnification lens

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13
Q

How do you calculate size of a real object?

A

How do you calculate size of a real object?

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14
Q

what is resolution?

A

the ability of a microscope to distinguish details of a specimen or sample.

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15
Q

magnification

A

the increase in size of an object when it is viewed through a microscope or some other optical device

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16
Q

how to work out mean length of cells

A

-place ruler on stage
-measure diameter/ width of field of view in mm
-focus on cells
-estimate/ count number of cells that sit (lengthwise) across a field of view.
divide diameter/width of field of view by estimated number of cells