world of microscope Flashcards
how do light microscopes work
they use a beam of light to form an image of the object
what magnification can light microscopes achieve
2000x
advantages of light microscopes
no radiation risk and are affordable
difference between light microscopes and electron microscopes
you can see live specimens on a light microscope but not an electron microscope as it uses a vacuum.
disadvantage of light microscope
low magnification and resolution ( make it difficult to see smaller objects)
importance of the development of electron microscopes
it enabled scientists to see and understand more about the subcellular structures in cells
How do electron microscopes work?
they use a beam of electrons to form an image
What magnification can electron microscopes achieve?
up to x2,000,000
What do transmission electron microscopes do?
they give 2D images with high magnification and resolution
What advantages are there to using electron microscopes?
higher magnification and resolution
What disadvantages are there to using electron microscopes?
they are large, very expensive and have to be kept in special temperature, pressure and humidity rooms
How do you calculate magnification with a light microscope?
multiply the eyepiece lens by the magnification lens
How do you calculate size of a real object?
How do you calculate size of a real object?
what is resolution?
the ability of a microscope to distinguish details of a specimen or sample.
magnification
the increase in size of an object when it is viewed through a microscope or some other optical device