WM: Chromatography Flashcards
1
Q
The mobile phase in TLC is…
A
A solvent (eg. Ethanol)
2
Q
How do you calculate the retardation factor for TLC?
A
distance travelled by spot
/
distance travelled by solvent
3
Q
The stationary phase in TLC is…
A
A thin layer of SILICA or ALUMINA fixed to a glass or metal plate
4
Q
How do you make colourless components visible in TLC?
A
Plates can have a fluorescent dye that shines when UV shines on it. The dots will cover the dye and won’t glow so you can see them.
You can also expose them to iodine vapour which sticks to the chemicals on the plate and show up as purple spots.
