wk 2 media prep Flashcards
TYPES OF CULTURE
- pure
- mixed
- stock
It is composed of only one specie
pure culture
It is composed of more than one species
MIXED CULTURE
Composed of several specie contained in a separate culture
medium
Used for academic or individual purpose
STOCK CULTURE
A nutrient material prepared for the growth of
microorganisms in a laboratory
CULTURE MEDIA
COMMON INGREDIENTS OF CULTURE MEDIA
- water, peptone, agar, blood serum, malt extract, yeast extract
yeast extract
Amino acids, growth factors,
inorganic salts
malt extract
Maltose, starchy, dextrins, and
glucose, 5% of proteins and
protein breakdown products,
mineral salts and growth factors
BLOOD/SERUM
Enriching Culture media
Digested protein
peptone
CLASSIFICATION OF CULTURE MEDIA
CONSISTENCY
COMPOSITION
FORMATION
FUNCTION
Nutrients are dissolved in water and bacterial growth is
indicated by a change in broth’s appearance from clear to
turbid
LIQUID MEDIA
Made by adding a solidifying agent to the nutrients and water
SOLID MEDIA
For special purposes where agar is added to media in
concentrations that are too low to solidify them
SEMI-SOLID MEDIA
Composition is exactly known, quantitatively. They are
chemically defined media prepared from pure chemical
substances. (e.g. SCA, MGM)
SYNTHETIC
Has a known composition only with approximation. The
complex medium contains ingredients in unknown
proportions (e.g. TSA, NA)
NON-SYNTHETIC
The medium test the ability of
organisms to utilize citrate as the
sole carbon source
Simmons Citrate Agar
This mineral medium is the first to
be published synthetic medium
for Listeria that has been shown
to be strictly carbon limited
Mineral Glucose Medium
General purpose, non-selective
growth medium that supports the
growth of most non-fastidious
bacteria
Trypto-casein Soy Agar
Simple medium that supports the
growth of many organisms
Nutrient Agar
Used for obligate intracellular bacteria (Rickettsia and
chlamydia)
TISSUE
ACCORDING TO FORMATION
Slant
Slant/Deep
Deep
Broth
Contains nutrients that support the growth of nonfastidious
organisms without giving any particular organism a growth
advantage
NUTRITIVE
When a substance is added to a liquid medium which inhibits
the growth of unwanted bacteria and favor the growth of
unwanted bacteria
ENRICHMENT
nutritive agar example
E.g. Nutrient Agar, Trypto-casein Soy Agar, SDA
ENRICHMENT agar example
E.g. Selenite F, Alkaline Peptone Water, Tetrathionate broth
Are prepared to meet the nutritional requirements of more
exacting bacteria by the addition of substances such as
blood, serum, or egg to the basal medium
enriched
enriched media example
E.g. BAP, CAP, LSS, BCYE
Contains one or more agents that are inhibitory to all
organisms except those “selected” by the specific growth
condition or chemical
SELECTIVE
selective agar example
E.g. MAC, TCBS, LJ, PEA, DCA, MTM
Employ some factor that allow colonies of bacterial species
or type to exhibit certain metabolic or culture characteristics
that can be used to distinguish them from other bacteria
growing in the same plate
DIFFERENTIAL
DIFFERENTIAL MEDIA EXAMPLE
E.g. MAC, BAP, HEA, XLD
Is a holding medium designed to preserve the viability of
organisms by maintaining moist environment and giving
nutrients but will not allow multiplication
TRANSPORT MEDIA
TRANSPORT MEDIA EXAMPLE
JEMBEC/TRANSGROW, Stuarts transport medium,
Cary-Blair
These media are used to grow anaerobic organisms and
contain reducing substances
ANAEROBIC
ANAEROBIC MEDIA EXAMPLE
E.g. Thioglycolate Broth, Cooked Meat Broth
Used for antibiotic susceptibility testing
MHA
shiny opaque, convex,
white colonies 2-3 mm in diameter
after 48hrs
S AUEREUS
Organisms that uses light as
energy source
PHOTOTROPH
Organisms that utilize the energy
produced by organic or inorganic
compound oxidation
CHEMOTROPH
TWO TYPES OF CHEMOTROPH
Autotroph/Litotroph AND Heterotroph/Organotroph
Organisms requiring oxygen for
growth
OBLIGATE AEROBES
Organisms NOT requiring oxygen
for growth
OBLIGATE ANAEROBES
Organisms not requiring air but
enhanced in its presenc
FACULTATIVE ANAEROBE
MICROAEROPHILE
Organisms requiring small
amount of air
Do not grow well but survives in
the presence of air
AEROTOLERANT
Grows between
pH 0 – 5.5
ACIDOPHILIC
Grow between 8.5 – 11.6
BASOPHILIC/ALKALOPHILIC
Grows between
pH of 7.0 – 7.5
NEUTROPHILIC
