aseptic Flashcards
procedures that is performed
under sterile conditions
ASEPTIC TECHNIQUE
triple packing system
. (container, yellow hazard
bag, and then zip lock)
goal of containers in the triple packaging system
1st container - holds sample
second container - leakage proof
third container - maintain viability
BLOOD CULTURE
ANTISEPTIC
Alcohol - lodine - Alcohol
anticoagulant used for blood culture
Anticoagulant: 0.025% SPS (sodium polyanethol
sulfonate)
o Neutralize bactericidal effect of human serum
o Prevents phagocytosis
o Antibiotic removal capability. 2 sites are usually
o requested. 2 sets are recommended
o Common antibiotics like macrolides.
SPS (ANTICOAGULANT)
BLOOD CULTURE BOTTLES volume
o 0.5-2 milliliter infants (per bottle) 1-4mL (per set)
o 1-5 milliliter children (per bottle)2-10 mL(per set)
o 5-10 milliliter adult (per bottle) 10-20mL (per set)
for respiratory culture Cultures must include
anaerobic condition for beta
streptococcus
THROAT
o Group A Streptococcal Infection group A strep pygenes.
o Oral thrush – Candida albicans
o Epiglottitis - Haemophilus influenzae type B
o Oral gonorrheae - Neisseria gonorrheae
o You should have a transport media
NASOPHARYNX
o Pertussis- B. pertussis
o Middle ear infection by Moraxella katalis or
Haemophilus Influenzae
o Staphlycoccus aureus or Neisseria meningitidis
carrier
o MRSA – Methicillin resistance Staph Aureus or
carriers of Neisseria Meningitidis.
o Usually requested for aerobic organism such as
Bordetella pertussis, Moraxella, Hemophilus influenzae
Specimen with too many EC/few PMN are not
suitable for culture
SPUTUM
Usually 2-3 specimen in consecutive days.
SPUTUM
URINARY TRACT INFECTION
o (Cystitis, urethritis, pyelonephritis,
glomerulonephritis)
o For UTI, pag makati yung genetals mo okay
kapa, pataas yan. Till kidney.
REQUIRED VOL FOR CATHETERIZED SAMPLE
15ML
o Recommended specimen FOR GASTROINETSTINAL
3 consecutive days.
3RD or 4th puncture in between of the 3 lumbar
vertebrae
CEREBSOSPINAL FLUID
HOW MUCH VOLUME FOR CSF
3-4 BOTTLES
3 REC
CSF WHEN TO INCUBATE AD CULTURE
Examine immediately or incubate in 37 degC for no longer than 6hours it must be incubated within 3 hours then culture agad.
GENITAL TRACT
o STI’s include infection caused by: Neisseria
gonorrheae, HSV-2, Clamydia trachomatis,
Trichomonas vaginalis, Gardnerella vaginalis,
Candisa albicans
o Sample: Male: urethral discharge, Urethral swab
(2-4 millimeters ipapasok sa penis 5x rotate)
o Sample Female: Urethral Discharge, Cervical swab
- Result from animal/human nites, ulcer,
burn, traumatic wounds
EXOGENOUS
- attributed to indigenous bacteria
ENDOGENOUS
Transport Condition
TRANSPORT Immediately at ROOM
TEMP
Ear,
Corneal Scrapings,
Foreign
bodies, gastric aspirate,
Suprapubic aspirate of
Urine,
Prostatic samples
Transport Condition
Within 15 minutes at
ROOM TEMP
CSF
Transport Condition
Within 2 hours at ROOM
TEMP
Blood or bone marrow
Transport Condition V
Immediately at 4 degC
Gastric biopsy
Transport Condition
Within 2 hours at 4 degC
Indwelling Catheter,
Straight
Catheter
maintains the appropriate colony
counts for urine speciements at room
temperature
BORIC ACID
absorbs fatty acids that may inhibit the
growth of fastidious bacteria
CHARCOAL
TRANSPORT MEDIA
Stool: cary-blair (cb);
viral: stuart medium (sm);
respiratory: amies;
gonococcus:
jembec/transgrow
SPECIMEN STORAGE
REFRIGERATOR TEMPERATURE (4°C)
o Urine, Stool, Sputum, Swabs, and Viral
Specimen
SPECIMEN STORAGE
INCUBATOR (35°C)
o CSF for 6 hours
SPECIMEN STORAGE
AMBIENT TEMPERATURE (22 TO 25°C)
Anaerobic culture, Sterile Body Fluids, Genital
Specimens, and Corneal/Ear Swab
SPECIMEN STORAGE
FREEZER TEMPERATURE (-20 TO -70°C)
o Serum, Tissue, Stool (C. difficile)
SPECIMEN PRIORITIZATION
CRITICAL/INVASIVE
Amniotic Fluid,
Blood, Brain,
CSF,
Heart Valves,
Pericardial
fluid
SPECIMEN PRIORITIZATION
UNPRESERVED
Bone, Feces,
Sputum,
Tissue, Other
body fluids not
listed unde
r
SPECIMEN PRIORITIZATION
QUANTITATION
REQUIRED
Urine,
Catheter Tip,
Tissue
PRESERVED
Urine, Stool,
and Swabs in
Transport
Media
Routinely used method to isolate bacteria
STREAK CULTURE
streak o Used for Isolation
QUADRANT STREAK
streak used for bacteriophage typing or antibiotics
sensitivity testing
LAWN STREAK
streak used in the preparation of bacterial
antigens and vaccines
LAWN STREAK
is made in tubes containing agar
slope/slant
Stroke culture
Provide a pure growth of bacterium for slide
agglutination and other diagnostic test
stroke
Prepared by puncturing a suitable medium
gelatin or glucose agar with long, straight,
charged wire
STAB CULTURE
Used to demonstrate gelatin liquefaction or to
study oxygen requirement of bacteria
STAB CULTURE
