WK 1 LAB Flashcards
AUTOCLAVE USE
STERILIZATION
AUTOCLAVE USES IT FOR SEALING AND CREATING PRESSURE
RuBBER GASKETS
what water is used in autoclave
distilled or deionized water
to achieve complete sterilization in an autoclave what sjhould be the stats
121C, 15psi for 15 mins
used to create the mold
used in this machine.
petri dish or tubes
most commonly used biosafety cabinet
class 2
bsc for very fatal organism
class 3
USED TO CONFINE DROPLETS OR BACTERIA
BSC
FILTER OF BSC
HEPA (high-efficiency particulate air)
filter and ULPA (Ultra low penetration air
filter air coming inside OF BSC
GRILLS
Used to provide optimum temperature for
growtH
INCUBATOR
to circulate hot air to dry plates
paglabas kasi sa autoclave basa pa sample
HOT AIR OVEN
FAN
HOT AIR OVEN STERILIZING SETTING
- Sterilizing setting 160-180c for 2 hrs
- Oxidizes constituents of bacterial cell
HOT AIR OVEN
Used for stabbing culture and detecting
bacterial motility
INOCULATING LOOP OR NEEDLE
Need to be disinfecter then autoclaved before
throwing it out
INOCULATING LOOP OR NEEDLE
- For mixing liquids
VORTEX MIXER/SHAKER
as cap-like depression in the top center
where the tubes are placed
VORTEX/MIXER
Moves in a dieratory motion affecting the
thorough mixing of what is placed inside the
test tube. speed can be adjusted
Vortex mixer/shaker
- Melts media, solution, and sample
Water bath
- Heats or melts sample and reagent
Heating mantle
For analyzing fluorescent material, especially
for spotting spots thin layer chromatography
UV VIEWING CABINET
Has a viewing port to avoid exposure to eyes
in the radiotion
UV VIEWING CABINET
No filter, confinement only though aerosols
and droplets might still leak outside
INOCULATION CHAMBER
Used for testing pH for culture medium. If its
too acidic, organisms wont grow h
PH METER
ALKALOPHILE EXAMPLE
CHOLERA
ACIDPHILE
enicyobactoprior
- Counting microbial colony
Colony counter
CFU
colony forming units
slows down the
metabolism of bacteria
REF
Sterilization, heat fixation of smear,
inoculation,transferring and plating culture,
etc
BUNSEN BURNER
- Measuring sizes of bacterial cell
Stage ocular micrometer
- Can either be digital or triple beam balance
Weighing balance
3 TYPES OF THERMOMETER
- Probe-type
- Digital
- Mercury
- Testing for coliform c water
MEMBRANE FILTER
- For sterilization liquids
MEMBRANE FILTER
Principle of microscopy
- Making small things visible
- Simplest microscope is
magnifying glass
HIGHEST MAGNIFICATION
1000X
TOTAL MAGNIFICATION
- Eyepiexe x objective lens
Ability of lens to distinguish 2 points at
separate
Resolution
RESOLVING POWER
READ
DIameter of the circle of light that you see
when working at a microscop
GIELD OF VIEW
MICROSCOPE THAT - Illuminates from light
BRIGHT FIELD
Has a special condenser diaphragm
that occludes direct light
DARK-FIEKLD
Ring-shape condenser shifts light so
theres an exchange of intensity in light
(has contrast making you see better
than in bright field)
PHASE CONTRAST
Best for analyzing urine and body
fluids
PHASE COCNTRAST
- Uses electron beams and magnets
ELECTRON MICROSCOPE
More powerful because it has shorter
waves of light
ELECTRON MICROSCOPE
Use chloroform light para maglow
yung reagent
FLOURESCENT MICROSCOPE
- Uses uv or fluorescent light
FLOURESCEWNT MICROSCOPE
- Uses uv or fluorescent light
LENS AND OBJECTIVE
4 OBJECTIVES
● Scanning Objective Lens
(4x)
● Low Power Lens (10x)
● High Power Lens (40x)
● Oil Immersion Lens (100x
LOBGEST OBJ
OIL IMMERSION
SHORTEST
SCANNER
- Focuses light
CONDENSER
- used so that there is no stray light
IMMERSION OIL
Buksan sa dimmer bg so that light going
upwards can be regulated
Iris diaphragm
