Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

BACTE LAB > WK 1 LAB > Flashcards

WK 1 LAB Flashcards

1
Q

AUTOCLAVE USE

A

STERILIZATION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

AUTOCLAVE USES IT FOR SEALING AND CREATING PRESSURE

A

RuBBER GASKETS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what water is used in autoclave

A

distilled or deionized water

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

to achieve complete sterilization in an autoclave what sjhould be the stats

A

121C, 15psi for 15 mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

used to create the mold
used in this machine.

A

petri dish or tubes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

most commonly used biosafety cabinet

A

class 2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

bsc for very fatal organism

A

class 3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

USED TO CONFINE DROPLETS OR BACTERIA

A

BSC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

FILTER OF BSC

A

HEPA (high-efficiency particulate air)
filter and ULPA (Ultra low penetration air

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

filter air coming inside OF BSC

A

GRILLS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Used to provide optimum temperature for
growtH

A

INCUBATOR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

to circulate hot air to dry plates
paglabas kasi sa autoclave basa pa sample

HOT AIR OVEN

A

FAN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

HOT AIR OVEN STERILIZING SETTING

A
  • Sterilizing setting 160-180c for 2 hrs
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q
  • Oxidizes constituents of bacterial cell
A

HOT AIR OVEN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Used for stabbing culture and detecting
bacterial motility

A

INOCULATING LOOP OR NEEDLE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Need to be disinfecter then autoclaved before
throwing it out

A

INOCULATING LOOP OR NEEDLE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q
  • For mixing liquids
A

VORTEX MIXER/SHAKER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

as cap-like depression in the top center
where the tubes are placed

A

VORTEX/MIXER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Moves in a dieratory motion affecting the
thorough mixing of what is placed inside the
test tube. speed can be adjusted

A

Vortex mixer/shaker

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q
  • Melts media, solution, and sample
A

Water bath

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q
  • Heats or melts sample and reagent
A

Heating mantle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

For analyzing fluorescent material, especially
for spotting spots thin layer chromatography

A

UV VIEWING CABINET

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Has a viewing port to avoid exposure to eyes
in the radiotion

A

UV VIEWING CABINET

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

No filter, confinement only though aerosols
and droplets might still leak outside

A

INOCULATION CHAMBER

25
Q

Used for testing pH for culture medium. If its
too acidic, organisms wont grow h

A

PH METER

26
Q

ALKALOPHILE EXAMPLE

A

CHOLERA

27
Q

ACIDPHILE

A

enicyobactoprior

28
Q
  • Counting microbial colony
A

Colony counter

29
Q

CFU

A

colony forming units

30
Q

slows down the
metabolism of bacteria

A

REF

31
Q

Sterilization, heat fixation of smear,
inoculation,transferring and plating culture,
etc

A

BUNSEN BURNER

32
Q
  • Measuring sizes of bacterial cell
A

Stage ocular micrometer

33
Q
  • Can either be digital or triple beam balance
A

Weighing balance

34
Q

3 TYPES OF THERMOMETER

A
  1. Probe-type
  2. Digital
  3. Mercury
35
Q
  • Testing for coliform c water
A

MEMBRANE FILTER

36
Q
  • For sterilization liquids
A

MEMBRANE FILTER

37
Q

Principle of microscopy

A
  • Making small things visible
38
Q
  • Simplest microscope is
A

magnifying glass

39
Q

HIGHEST MAGNIFICATION

A

1000X

40
Q

TOTAL MAGNIFICATION

A
  • Eyepiexe x objective lens
41
Q

Ability of lens to distinguish 2 points at
separate

A

Resolution

42
Q

RESOLVING POWER

A

READ

43
Q

DIameter of the circle of light that you see
when working at a microscop

A

GIELD OF VIEW

44
Q

MICROSCOPE THAT - Illuminates from light

A

BRIGHT FIELD

45
Q

Has a special condenser diaphragm
that occludes direct light

A

DARK-FIEKLD

46
Q

Ring-shape condenser shifts light so
theres an exchange of intensity in light
(has contrast making you see better
than in bright field)

A

PHASE CONTRAST

47
Q

Best for analyzing urine and body
fluids

A

PHASE COCNTRAST

48
Q
  • Uses electron beams and magnets
A

ELECTRON MICROSCOPE

49
Q

More powerful because it has shorter
waves of light

A

ELECTRON MICROSCOPE

50
Q

Use chloroform light para maglow
yung reagent

A

FLOURESCENT MICROSCOPE

51
Q
  • Uses uv or fluorescent light
A

FLOURESCEWNT MICROSCOPE

52
Q
  • Uses uv or fluorescent light
A

LENS AND OBJECTIVE

53
Q

4 OBJECTIVES

A

● Scanning Objective Lens
(4x)
● Low Power Lens (10x)
● High Power Lens (40x)
● Oil Immersion Lens (100x

54
Q

LOBGEST OBJ

A

OIL IMMERSION

55
Q

SHORTEST

A

SCANNER

56
Q
  • Focuses light
A

CONDENSER

57
Q
  • used so that there is no stray light
A

IMMERSION OIL

58
Q

Buksan sa dimmer bg so that light going
upwards can be regulated

A

Iris diaphragm

59
Q
A

BACTE LAB (4 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions