Willmott 5 enzymes Flashcards
1
Q
ENZYMES:
A
proteins which catalyse biological reactions, unchanged, accelerate reactions 106fold by Highly specific => active site -> arrangement of AAs
2
Q
2 Factors determine appearance of product
A
Thermodynamics & kinetics
3
Q
V=
A
rate of reaction
4
Q
[S]=
A
conc. Of substrate
5
Q
Vmax=
A
max rate of reaction
6
Q
Km =
A
substrate conc at which reaction proceeds ½ of max rate (Vmax) units = Molar
7
Q
MICHAELIS-MENTEN Eq
A
v= Vmax [S]/ Km +[S]
8
Q
MICHAELIS-MENTEN Eq
graph
A
Initial rate against substrate conc
Vmax is hyperbola
Km is Vmax/2
9
Q
Lineweaver - Burk Plot
graph
A
y=0 = 1/Vmax X=0 = -1/Km Slope = Km/Vmax
10
Q
Importance of Km
A
The lower the Km the higher the affinity of the enzyme to substrate
