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Micro exam 2 > Wet lab > Flashcards

Wet lab Flashcards

1
Q

Bacteroides fragilis

A

GN pleomorphic rods that stain pale

  • grows in 20% bile
  • grows on KV
  • resistant to K,V,C
  • cat pos
  • indole neg
  • tan raised colonies
  • issues below the diaphram
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2
Q

Prevotella

A

Gram neg coccobacilli

  • susceptible to colistin
  • black brown pigment on KV
  • Cat neg
  • indole neg
  • brick red flouresence
  • head, pulmonary and neck injuries
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3
Q

Porphyromas

A

head, neck ,pulmonary infections

  • Gram neg coccobacilli
  • sensitive to Van. so does not grow on KV
  • black brown pigment on brucella
  • cat and indole neg
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4
Q

fusobacterium

A

pulmonary brain, oral infections

  • Colistin and kanamycin susceptible
  • long slender GNRs with tapered ends
  • does not grow on KV
  • bad odor and green breadcrumb like colonies on Brucella, VERY SENSITIVE TO OXYGEN
  • Cat neg
  • indole positive
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5
Q

Clostridium perfringens

A

gas- gangrene

  • powerful enterotoxin and food posioning
  • large box car GPRs variable spores
  • can appear GN
  • double zone hemolysis
  • reverse camp positive ( pac man )
  • spore formers`
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6
Q

Vibrio

A

gram neg curved rods

  • likes brackish/salt water
  • can get by eating undercooked seafood or ingesting water
  • OX positve
  • indole positive
  • beta hemolytic
  • grow on TCBS media- has lots of salt
  • use alkaline peptone water to enhance growth
  • produces enterotoxin that produces rice water dirrahea
  • FERMENTS GLUCOSE
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7
Q

Aeromonas

A

brackish/ low salt water

  • hydrophillia
  • ferments glucose
  • ox pos
  • indole pos
  • beta hemolytic
  • can diff. from vibrio because it will not grow on TCBS
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8
Q

Camplobacter

A
  • found in animals-poultry
  • common cause of dirrahea
  • grows on campy plate at 42C in microphillic conditions
  • gull wing GNR
  • grey/pinkish/yellow mucoid colonies
  • cat positive
  • hiperhydratre pos
  • Jejubi is susceptible to nalidixic
  • fetus is susceptible to ceph
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9
Q

pseudomonas aeruginosa

A

commonly isolted NF-GNR

  • found in water, shower heads, …
  • common cause of nosocomial infections
  • is a very significant pathogen in burn and CF pts
  • causes swimmers ear
  • leading cause of hospital aquired pneumonia
  • OX positive
  • blue/green colonies ( need specific conditions)
  • fruity odor
  • ID with comerical systems
  • non lac. ferm
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10
Q

Stenotrphomonas Maltophillia

A
  • found in hospitals and on equipment
  • GNR
  • NFL
  • ox neg
  • purple/yellow/green conditions
  • smells like ammonia
  • ID with comerical equipment
  • use bactrum, sxt, or trimethalsulfa
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11
Q

Actinobacter

A
  • hospital infections
  • GNR but looks cocci
  • ox neg
  • easy to id
  • resistant to many bacteria
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12
Q

burkholdaria cepaciae

A

plant pathogen, found in hospitals

  • very important pathogen in CF patients
  • ox pos
  • OF glucose pos
  • ons on OFBL medium
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13
Q

Pasteurella

A
  • found in cats and dogs
  • humans infected by bites
  • can affect immune compromised
  • GNR that grows well on BAP/CHOC but not mac
  • requires CO2
  • OX/indole positive
  • responds well to penicillin
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14
Q

Hemophilus Influenzae

A

GNCB

  • encapsulated strains can be life theatening( meningitis, epiglottitis, celluitis, …)
  • non encapsulated more localized infections, ottis media, sinusitis
  • test susceptibility to Beta lactams, ampicillin
  • only grows on blood with staph.
  • X and V factors
  • needs CO2
  • ox positive
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15
Q

legionella

A

water bug in hospitals, hotels, ….

  • cause on legionares disease
  • faint staining long thin rods
  • strict anaerobes
  • grows on chocolate
  • BYCE to culture
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16
Q

Neisseria gonorrhoeae

A

GN DC

  • never normal flora
  • ox pos
  • cat pos
  • sensitive to drying /cold
  • only grows on choc and mtm
  • ferments glucose
  • pen resistnace
17
Q

Neisseria meningitidis

A 
normal inhabitant of upper respiratory tract
- leading cause of meningitis
- spread by aerosolization
- Cat positive
- grows on BAP/CHOC/ MTM
- ferments glucose and maltose
-
18
Q

Moraxella catarrahlis

A

normal inhabitiant of upper resp. tract

  • GNDC
  • OX pos
  • CAT pos
  • will grow on Blood and choc
  • dnase positive
  • producees burate esterade
  • determine ampicillin sensi
19
Q

Listeria monocytogenes

A
  • only GPR of medical importance
  • causes bacteremia and meningitis
  • can be passed from mother to fetus
  • GPR -small
  • grows well on BAP and CHOC
  • looks lihe group B strep on B plate
  • CAT POS
  • esculin pos
  • camp test positive
  • non spore former
  • tumbling motility
20
Q

Corynebact

A 
dipthroirds of skin flora
 grows on tinsdale, loeffer, and cystine...
- club shaped GPR
- non hem. 
- lippophillic
- cat pos
- esculin neg
- non spore former
21
Q

Bacillius

A

large GPR wl square ends

  • form spores
  • decolorize on gs
  • obligate anerobes
  • cat pos
  • ox pos
  • motile
  • beta hemolytic
  • grow on blood and chocolate
22
Q

lactobacillus

A

highly pleomorphic

  • colony types variable
  • alpha hem grows on blood/ choc
  • can be confused with viridands
  • lac is vanc resistance
  • cat neg
  • non motile
  • nonspore forming
23
Q

erysipelothrix rhisiopathiae

A

normal flora of animals

  • get from bites
  • causes bacteremia
  • cat neg, non motile, non- spore former
  • H2S positive
24
Q

group characteristics of Anaerobes

A
  • almost all are endogenous
  • susceptibility patteren to colistin, kanamycin, vancomycin
    -slow growers
    -
25
Q

non-fermenting GNR

A
  • non-spore forming
  • do not ferment glucose
  • saprophytic do not use glucose at all
  • most will grow on a blood or mac plate
  • aerobicc
  • ox pos
  • not part of normal flora
  • found in water/soil
  • nosocomial infections
  • have a high level of antibiotic resistance
26
Q

Fastidious gram negatives

A 
required enriched medium
- most ferm glucose
- ox positive
- all require CO2
- slow growers
-

Micro exam 2 (6 decks)

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