Anaerobic bacteria

Question 1

Obligate aerobic bacteria

Answer 1

need oxygen

- grow at the top of the tube

Question 2

obligate anaerobe

Answer 2

avoids oxygen

- grows at the bottom of the tube

Question 3

facultative bacteria

Answer 3

can grow in either but will chose oxygen because it is more energy effective

Question 4

microphiles

Answer 4

needs oxygen but at low concentrations

Question 5

aerotolerant

Answer 5

oxygen does not affect them grows through out the tube

Question 6

it is unacceptable to use anaerobic culture for

Answer 6

superficial wounds, vaginal/urethral swabs, respiratory specimens, stool, urine( except subpubic aspirate)

Question 7

do not use swabs because

Answer 7

swabs are sub optimal because drying swabs adhere to the swab and are subject to contamination

Question 8

KV agar

Answer 8

contains Kanamycin, vancomycin, laked blood

- for bacteroides and provetella

Question 9

brucella

Answer 9

all anaerobes

• sheep blood, vit K, hemin
• enriched medium

Question 10

PRAS

Answer 10

anaerobic condition system that is made of sterilized tubes that you innoculate with a syringe

• total anaerobic system
• EXPENSIVE

Question 11

BD bio bag

Answer 11

disposable chamber

• potassium borohydride and sodium bicarbonate combined with a palladium catalyst
• resazurin indicator
• expensive and takes up room

Question 12

Jars

Answer 12

gas pouch

• add water to combine with sodium borohydrate
• catalyst
• anaeropack system
• no water needed just add the pack
• • disadvantage, takes up a lot of space and cant add plates after activator is in

Question 13

Anoxomat

Answer 13

hooks up to a computer that allows you to add plates whenever needed and the system will bring back to the right conditions

Question 14

Glove box

Answer 14

completely anaeronic conditions

Question 15

Bacteroides

Answer 15

• isolate of the intrabdominal tract, respiratory tract, gential tract, brain, skin, soft tissue infections
• resistant to many antibiotics( resistant to Penicillin)
• KV agar- tan/red round colonies
• GRAM NEGATIVE COCCOBACILLI/RODS
• catalase positive
• indole negative
• bile sensitive

Question 16

Fusobacterium

Answer 16

fusiform, slender, long GNRs, tapered ends

• breadcrumb colonies green on brucella
• no growth on KV
• catalase neg
• indole positive
• rancid odor
• can cause pharyngitis
• associated with jugular vain thrombosis

Question 17

prevotella melaninogenica/ porohyromonas

Answer 17

Gram neg coccobacilli

• red flouresence under UV
• Cat neg
• indole pos
• isolate from head, neck, and pulmonary infections
• Prev. : Grows on KV suseptible to colistin
• Por.: fors not grow on KV, susceptible to vancomycin

Question 18

Clostridium Perfringens

Answer 18

• causes myonecrosis
• spores enter the wound
• box car GNRs
• reverse camp positive ( pacman)
• double zone hemolysis
• lechtinase positive

Question 19

Clostridium septicum

Answer 19

associated with bowel cancer and leukemia

• reverse camp negative
• swarming colonies on a brucella
• GNR: subterminal spores

Question 20

C. Difficile

Answer 20

nosocomial infection

• survives and multiplies
• diagnose with an EIA
• or PCR

Question 21

propionibacterium

Answer 21

Gram positive rods/ looked liked squished spiders

• aerotolerant accepte Proponicus
• acnes: found on skin indole and cat neg
• proponicus, and granulomatous; indole neg and cat pos
• mostly contaminants
• found in immunosuppression, diabetes, and implantation of foreign bodies

Question 22

actinomyces

Answer 22

• many species are aerotolerant
• normal flora of GI/GU tract, mouth
• found in sulfur granules
• branching pleomorphic rods
• modified acid fast negative
• grows on brucella
• ID by mass spec