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Microbiology Exam 1 > Wet lab > Flashcards

Wet lab Flashcards

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Biology 101 flashcards
1
Q

Gram positive, Cat pos organisms

A
  • GPC
  • Catalase positive
  • aerobes and facultative anaerobes
  • only Staph aureus is clinically significant
  • humans are the natural reservoir
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2
Q

S. Aureus

A

GPC , Cat pos

  • have many virulent factors: Catalase, Coagulase, DNAse, hyaluronisase…
  • usually skin and soft tissue infections
  • can cause bactermemia, sepsis, ostomylitis
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3
Q

Coag Negative staph

A
  • coag and DNAse negative
  • Epidermitis Saprohyticus, Lugdunensis
  • form biofilms
  • saprophyticus is resistant to novobiocin
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4
Q

Catalase test

A

use hydrogen peroxide to determine if the organism has catalase

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5
Q

Coagulase

A

is used to differentiate S. Aureus from other coag negative staph.
- Staph produces free and bound coagulase
- mix with rabbit plasma to observe clumping
-

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6
Q

Mannitol Salt agar

A

is selective and differential media

  • used for the isolation and presumptive ID of S. Aureus
  • SA is able to ferement mannitol turns media to yellow
  • has lots of salt
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7
Q

Novobiocin

A

make a .5 mcfarland standard

  • streak a lawn and place a novo. disc one the media
  • Saprophyticus is resistant
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8
Q

A Disc

A

Bactracin

  • streak for lawn
  • add disc
  • S. Pyogenes susceptible
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9
Q

CAMP

A

Streak S. Aureus down the center then close in the opposite directions streak other things

  • if postive the hemolysis will be arrow shaped
  • Group B strep
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10
Q

Optochin disc

A

P disc

  • used to determine S. Pnuemonia
  • > than 14mm for susceptible
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11
Q

Klebsiella

A

Lactose Ferm on MAC
encapsulated so mucoid colonies
- Pneumo - neg, Oxytoca- Pos

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12
Q

Proteus

A

swarmer

  • non lac ferm
  • mirabilas- neg, virgalis - pos
  • phenolalanine deaminase pos
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13
Q

MAC plate

A
  • uses bile salts and crystal violet in the medium to encourage growth of GNR
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14
Q

MRVP

A

1st part: add methyl red to indicate acid end products fermentation glucose
- VP: detects neutral end products of glucose fermentation

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15
Q

Oxsidase

A

swab colony

  • add oxidase reagent blue is positive
  • pseudomonas: positive
  • E. Coli negative
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16
Q

indole

A

the ability of the oranism to produce indole

  • add Kovacs reagent to tube and a red color is positive
  • Rapid indole: swab colony, indole positive
17
Q

Nitrate reduction

A

nitrate to nitrite
- add solutions a and B
- if red positive
- if negative add zince to see if it will reduce nitrite to nitrate if red on second time its negative
Tube is a nitrate broth with a durham tube

18
Q

Citrate

A

to see if the organism uses urea as its carb source and ammonium as its nitrogen source

  • streak a citrate agar (do not stab)
  • positive (K. pneumonia) green to blue
  • negative: E. Coli
19
Q

Urea

A

ability to produce urease

  • streak urea slant
  • magenta color change positive(K. pneumonia, P. Mirabilis)
  • negative E. coli
20
Q

Decarboxylase tests

A

the ability to decarboxylate AA to amine form,

  • base has only glucose,then a lysine and ornathine broth =
  • if they are purple they are positive, if yellow negative
21
Q

TSI

A

differentiates GNR based on Carb fermentation

  • glucose feremnted in slant and butt
  • Lactose and Sucrose can be fermented in the Slant
  • K/K( red/red)= no carb fermenetaion
  • A/A(yellow/yellow)= glucose and sucrose/lactose ferm
  • K/A (red/yellow)= only glucose ferm
  • black butt= glucose fermented/h2s
22
Q

LIA

A

ability to decarboxylate or deaminate lysine and produce H2S

  • used to screen suspicious shigella/ Salmonella
  • in the butt glucose fermentation and lysine decarbox.
  • in slant demainates
  • K/K(purple/purple)= lysine is decarbylated
  • K/A(purple/yellow= (lysine not decarbox)
  • R/A(red/yellow)= lysine deaminated
23
Q

MIO

A

determines GNR motility,indole, and ornathine production

  • ornathine = purple throughout tube
  • motility- growth away from the slab line
  • indole= add kovacs reagent red is positive

Microbiology Exam 1 (7 decks)

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