Wendt's Anticoagulant Lectures

Question 1

What do serine proteases do?

Answer 1

Cleave clotting factors

Question 2

What do glycoproteins do?

Question 3

What does Ca²⁺ do?

Answer 3

Links certain factors to anionic lipids

Question 4

What do transglutaminases do?

Answer 4

Cross-link fibrins (Factor XIII)

Question 5

What does fibrinogen do?

Answer 5

Ultimately, the substrate protein for thrombin that polymerizes to form a clot

Question 6

Where are (most) clotting factors produced? What are the exceptions?

Answer 6

• All clotting factors (except vWF and factor VIII) are made in the liver
• vWF is made in the (sub)endothelium and megakaryocytes
• Factor VIII is also produced in the endothelium

Question 7

What factor outside the bloodstream does the extrinsic pathway rely on for activation?

Answer 7

Tissue factor

Question 8

Which pathway is initiated by contact with negatively-charged collagen of a diseased or injured vessel?

Answer 8

Intrinsic pathway

Question 9

All components in blood are part of which pathway?

Answer 9

Intrinsic pathway

Question 10

Which clotting factor does tissue factor bind to and activate? What happens after it’s activated?

Answer 10

Factor VII; once activated, binds to and cleaves factor X

Question 11

Explain thrombin’s role in the common pathway.

Answer 11

Thrombin converts fibrinogen into fibin, and activates factor XIII that cross-links fibrin to form a stable clot