Weeks 7-9

Question 1

What is batch effect?

Answer 1

Most common confounding factor, affected by time and who performs the tests.

Question 2

Stratification

Answer 2

Divide equally each type of subgroup between experimental groups.

Question 3

Quantile normalisation

Answer 3

Find top rank for each patient and find mean of top 3 across samples. Bring the same level value and then use the mean.

Question 4

General workflow of imaging?

Answer 4

Noise removal (background subtraction)
Segmentation (determine what we want to capture
Measurements
Post analysis
Machine learning

Question 5

Western blot

Answer 5

Stimulate w insulin, buffer then centrifugate at different speeds. Fractioning. Gel electrophoresis, then use antibody that will detect the reporter.

Question 6

Relative quantitation analysis

Answer 6

Compare abundance across samples

Question 7

Absolute quantitation analysis

Answer 7

Number of molecules

Question 8

Mass spectrometry

Answer 8

Light through inlet –> ion source –> mass analyser –> detector.

Question 9

Continuous electrospray ionisation (ESI)

Answer 9

High electrical field with liquid –> spray into fine droplets –> positive charge –> can be detected.