Week 10

Question 1

Metabolic labelling

Answer 1

Stable isotope labelled metabolite incorporated into protein. Mass tagging. Often arginase

Question 2

Chemical labelling

Answer 2

Incorporated via chemical reaction afterwards.

Question 3

Mean of measuring half-life easily?

Answer 3

Grow cell in medium-labelling tissue, then change to heavy isotope. They will convert slowly to completely heavy isotope - this rate is proportional to their half-life.

Question 4

GWAS

Answer 4

Large case/control pop –> blood samples –> SNP chips –> pop analysis –> Manhattan plot.

Question 5

Odds Ratio (OR)

Answer 5

(odds outcome occurs given exposure compared to odds outcome given no exposure).

Question 6

Whole genome/exon sequencing

Answer 6

Fragment DNA and hybridise. Wash and sequence captured DNA, allowing them for mapping, alignment and variant calling.

Question 7

Illumina sequencing

Answer 7

Fragment genome and put on polyA tails + labelled nucleotide. Hybridise to the primer. Each base has their own dye that can be identified for sequencing.