Week 9 - Particle Analysis Flashcards
1
Q
Side Scatter
A
Cell surface and internal complexity (granules, vacuoles)
Detector is off to the side
2
Q
Forward Scatter
A
Cell size/volume
Detector in line with light source
3
Q
Three Components of Flow Cytometry
A
- Fluidics
- Light Source
- Detectors
4
Q
Fluidics
A
Cells in a fluid suspension when injected
5
Q
Hydrodynamic focusing
A
Pressurized fluid focuses cell into a single file