Week 9 - Particle Analysis Flashcards

1
Q

Side Scatter

A

Cell surface and internal complexity (granules, vacuoles)

Detector is off to the side

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2
Q

Forward Scatter

A

Cell size/volume

Detector in line with light source

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3
Q

Three Components of Flow Cytometry

A
  1. Fluidics
  2. Light Source
  3. Detectors
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4
Q

Fluidics

A

Cells in a fluid suspension when injected

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5
Q

Hydrodynamic focusing

A

Pressurized fluid focuses cell into a single file

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