Week 9 - Enzymes and Transporters

Question 1

What is the Michaelis-Menten Kinetics equation?

Answer 1

• V=V_max[S]/K_m+[S]
• K_m is a constant
• inversely proportional to affinity

Question 2

How are enzymes classified?

Answer 2

• oxidoreductases
• oxidation/reduction reactions
• transferases
• transfer functional groups
• hydrolases
• hydrolysis
• lysases
• add/remove atoms to form double bonds
• isomerases
• rearrange atoms within molecule
• ligases
• joining of molecules

Question 3

What are the types of enzyme inhibitors?

Answer 3

• competitive
• increases K_m
• same V_max
• non-competitive
• lowers V_max
• uncompetitive
• bind to enzyme-substrate complex to stabilise it
• prevents product formation
• increasing [S] enhances inhibition
• lowers V_max and K_m