Week 9

Question 1

1.You would like to express a glycosylated protein in a heterologous system, which one of the following would NOT be suitable for this

Answer 1

E.coli

Question 2

2.Which one of the following vectors is incorrectly paired with its use?

Answer 2

Baculovirus vectors – mammalian transformation

Question 3

3.What is a bacmid?

Answer 3

a shuttle vector that replicates in E. coli and insect cells

Question 4

4.Which TWO of the following are NOT used as selectable markers to maintain mammalian shuttle vectors within mammalian cells?

Answer 4

Rifampicin and ampicillin

Question 5

5.Stem cells that can differentiate into all cell lineages are called?

Answer 5

totipotent

Question 6

6. What is NOT one of the three principle methods of making a transgenic mammal?

Answer 6

transposons