Practicals Flashcards
Even with very proficient pipette technique what error can you expect to make every time you dispense?
2-3%
Eppendorf is a brand name, what is the correct name for an “Eppendorf” tube?
Micro-centrifuge tube
Eppendorf is a brand name, what is the correct name for an “Eppendorf” tube?
Micro-centrifuge tube
This is a micro-centrifuge. The blue circles represent positions were micro-centrifuge tubes have been inserted.
You have 3 more tubes to put into the centrifuge.
There are many ways to balance this but which of the below is a valid way.
Adding tube to:
B, E & G
After centrifugation the tubes from above look like the attached image. Which part is the supernatant?
The blue liquid
Feedback: The supernatent denotes the liquid lying above a solid residue after crystallization, precipitation, centrifugation, or other process.
You are going to load an agarose gel with a ladder and 2 different DNA samples. You intend to load each sample with a final volume of 12 µl of the DNA sample, how much 4x loading dye should you mix with your sample?
3µl
The following is an agarose gel, it shows the result of an attempt to amplify a DNA barcode (rDNA) of 4 nematodes by PCR.
Select below all the statements that are TRUE
There were no missing components within the PCR mix as the positive control has worked
We have amplified a DNA product of approximately the same size in every reaction
Feedback: There is a band in the negative control, which suggests their has been contamination with DNA. This means we do not know if the contaminated nematode DNA was amplified an appeared in the wells for nematodes 1-d. Perhaps, this is the desired nematode, the contamination or both. We know that the enzymes, primers, buffer, dNTPs etc are all in the PCR mix as DNA was successfully amplified. You would normally look to the positive control to be an indicator of this. We can see the DNA product is in line with ladder in the same place.
