week 8: protein translation and analysis

Question 1

the components involved in protein translation

Answer 1

• ribosome : site of translation. har large and small subunits that come together
• rRNA: part of ribosome. 16S in pro., 18S in euk.
  -tRNA: brings specific amino acids to ribosome
  -mRNA: the transcript from which protein is translated from

Question 2

how is translation initiated in prok vs euk

Answer 2

In prok., 16S rRNA is bound by Shine Dalgano sequence. In euk., Kozak sequence bound by 18S rRNA

Question 3

the four level organisation of proteins

Answer 3

-primary: amino acids
-secondary: a-helices /B-sheets
-tertiary: 3D
-quarternary: more than one amino acid chain

Question 4

ways to analyse proteomes (all proteins encoded)

Answer 4

-2D-SDS-PAGE and mass spectrometry
- protein array

Question 5

ways to analyse small number of proteins

Answer 5

-ELISA
-1D-SDS-PAGE
-Western blotting