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BACTE LECTURE > WEEK 8 > Flashcards

WEEK 8 Flashcards

1
Q

appearance of enterobacteriaceae

A

gram negative bacilli

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2
Q

oxidase test principle

A

cytochrome oxidase

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3
Q

oxidase test indicator

A

indophenols blue

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4
Q

wire use in screening test of oxidase test

A

platinum wire

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5
Q

reagent of filter paper in oxidase test

A

Tetramethyl p-phenylene diamine dihydrochloride

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6
Q

oxidase positive color

A

bluish purple

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7
Q

oxidase positive organism

A

P. aeruginosa

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8
Q

oxidase negative color

A

colorless

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9
Q

oxidase negative organism

A

e. coli

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10
Q

nitrate reduction test principle

A

nitrate reductase

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11
Q

medium in nitrate reduction test

A

peptone broth

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12
Q

2 reagent in nitrate reduction test

A

Sulfanilic Acid & α-napthylamine

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13
Q

reducing agent in nitrate reduction test

A

zinc dust/powder

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14
Q

T/F: all enterobacteriaceae reduces nitrate into nitrite

A

true

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15
Q

product in nitrite reduction test

A

gas production / N2

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16
Q

3 reagent in nitrite reduction test

A

Sulfanilic Acid, Alpha-napthylamine, zinc dust

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17
Q

positive result in nitrite reduction test

A

colorless

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18
Q

positive result in nitrite reduction test organism

A

proteus mirabilis

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19
Q

pathogenicity test for enterobacteriaceae

A

ONGP test

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20
Q

enzyme in ONPG test

A

beta galactosidase

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21
Q

T/F all ONPG test positive are lactose fermenters

A

True

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22
Q

structure is almost the same with lactose

A

onpg disc

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23
Q

separate lactose into galactose and glucose

A

beta galactosidase

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24
Q

ONPG test positive color

A

yellow

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25
onpg test postive organism
E. coli
26
onpg test negative color
no change in color
27
onpg test negative organism
Salmonella typhirium
28
ONPG positive with beta galactosidase and permease
RLF
29
ONPG positive with galactosidase only
LLF
30
ONPG negative without galactosidase and permease
NLF
31
Detects the ability of Enterobacteriaceae to | decarboxylase amino acids
LYSINE, ORNITHINE, ARGININE TEST (LOA TEST)
32
loa test principle
remove carboxylic group
33
Lysine
Cadaverin
34
Putrecine
Ornithine
35
Arginine
Citrulline
36
LOA Test positive
purple
37
loa test negative
yellow
38
loa test medium
Moeller’s decarboxylase medium
39
loa test indicator
Bromcresol purple
40
To differentiate the different bacteria under Enterobacteriaceae family according to their ability to ferment different sugars
triple sugar ion
41
TSI medium
TSI Agar (Butt-slant Medium)
42
TSI pH indicator
Phenol Red
43
phenol red color in acidic environment
yellow
44
Ferrous sulfates + H2S
Black / Black precipitate
45
A/A = 2-3 sugars fermented - ?
lactose fermenters
46
? = glucose fermented – non lactose fermenters
K/A
47
K/K = ? – non fermenter organisms
no sugar fermented
48
Glucose + Lactose + Sucrose + Iron
KLIGLER’S IRON AGAR (KIA)
49
Glucose + Lactose
RUSSELL’S DOUBLE AGAR (RDA)
50
T/F: In TSI, glucose is seen in the butt of the medium
True
51
T/F: In TSI, lactose and sucrose is seen in the butt of the medium
False
52
lysine iron agar mediym
Moeller’s Medium
53
lysine decarboxylation positive result
Purple
54
Lysine Deamination positive result
Red
55
Indole from tryptophan due to tryptophanase
indole test
56
indole test medium
SIM medium
57
Confirmatory reagent in indole test
Kovac's/Erlich's reagent
58
what does SIM stand for
Sulfide Indole Motility Medium
59
positive result in indole test
ring formation
60
Filter paper strips impregnated with pdiaminocinnamaldehyde reagent use for anaerobic bacte
RAPID SPOT INDOLE TEST
61
opposite of Voges-Proskauer
Methyl Red test
62
principle of methyl red test
Mixed Acid Fermentation
63
indicator of methyl red test
Methyl red
64
Medium of methyl red test
MRVP medium
65
MRVP medium is also known as
Clark and Lubs medium
66
indole test positive color
RED
67
indole test positive organism
E. cloaceae
68
indole test negative color
yellow
69
indole test negtaive organism
E. coli
70
pH of E. coli
alkaline
71
Butylene Glycol of Glucose Fermentation
Voges-Proskauer
72
Voges-Proskauer also known as
Acetoin / Acetylmethylcarbinol
73
Voges-Proskauer confimatory reagents
Alpha naphthol 40% KOH for enteric Alpha naphthol in 40% KOH in Creatine
74
40% KOH for Enteric
Barrits method
75
Alpha naphthol in 40% KOH in creatine
Coblentz method
76
voges-proskauer positive result color
red
77
voges-proskauer positive result organism (KESH)
Kllebsiella, Enterobacter, Serratia, Hafnia
78
voges-proskauer negative result color
yellow
79
organism positive in Barrit's method
E. cloacae
80
organism negative in Barrit's method
e. coli
81
organism positive in Coblentz method
Streptococcus mutans
82
Principle: Utilize citrate as source of carbon and energy
Citrate utilization
83
Citrate utilzation medium
Simmon's citrate
84
Citrate utilzation indicator
bromthymol blue
85
bromthymol blue in acidic
green
86
bromthymol blue in alkaline
blue
87
end product of citrate utilzation
Sodium carbonate
88
citrate utilization positive organism
P. aeruginosa
89
end product: Ammonium carbonate
UREA HYDROLYSIS TEST (UREASE)
90
Urea hydrolysis test medium
Christensens agar
91
Urea hydrolysis test indicator
phenol red
92
Urea hydrolysis test positive color
RED/PINK
93
identification for vibrio cholera
String test
94
Medium of string test
0.5% sodium deoxycholate
95
Esculin HOH test positive result color
black
96
Esculin HOH test negative result color
yellow
97
Gelatin hydrolysis test positive result
gel liquefies
98
enzyme that destroys gelatin
gelatinase

BACTE LECTURE (3 decks)

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