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BACTE LECTURE > WEEK 8 > Flashcards

WEEK 8 Flashcards

1
Q

appearance of enterobacteriaceae

A

gram negative bacilli

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2
Q

oxidase test principle

A

cytochrome oxidase

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3
Q

oxidase test indicator

A

indophenols blue

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4
Q

wire use in screening test of oxidase test

A

platinum wire

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5
Q

reagent of filter paper in oxidase test

A

Tetramethyl p-phenylene diamine dihydrochloride

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6
Q

oxidase positive color

A

bluish purple

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7
Q

oxidase positive organism

A

P. aeruginosa

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8
Q

oxidase negative color

A

colorless

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9
Q

oxidase negative organism

A

e. coli

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10
Q

nitrate reduction test principle

A

nitrate reductase

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11
Q

medium in nitrate reduction test

A

peptone broth

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12
Q

2 reagent in nitrate reduction test

A

Sulfanilic Acid & α-napthylamine

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13
Q

reducing agent in nitrate reduction test

A

zinc dust/powder

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14
Q

T/F: all enterobacteriaceae reduces nitrate into nitrite

A

true

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15
Q

product in nitrite reduction test

A

gas production / N2

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16
Q

3 reagent in nitrite reduction test

A

Sulfanilic Acid, Alpha-napthylamine, zinc dust

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17
Q

positive result in nitrite reduction test

A

colorless

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18
Q

positive result in nitrite reduction test organism

A

proteus mirabilis

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19
Q

pathogenicity test for enterobacteriaceae

A

ONGP test

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20
Q

enzyme in ONPG test

A

beta galactosidase

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21
Q

T/F all ONPG test positive are lactose fermenters

A

True

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22
Q

structure is almost the same with lactose

A

onpg disc

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23
Q

separate lactose into galactose and glucose

A

beta galactosidase

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24
Q

ONPG test positive color

A

yellow

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25
Q

onpg test postive organism

A

E. coli

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26
Q

onpg test negative color

A

no change in color

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27
Q

onpg test negative organism

A

Salmonella typhirium

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28
Q

ONPG positive with beta galactosidase and permease

A

RLF

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29
Q

ONPG positive with galactosidase only

A

LLF

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30
Q

ONPG negative without galactosidase and permease

A

NLF

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31
Q

Detects the ability of Enterobacteriaceae to

decarboxylase amino acids

A

LYSINE, ORNITHINE, ARGININE TEST (LOA TEST)

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32
Q

loa test principle

A

remove carboxylic group

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33
Q

Lysine

A

Cadaverin

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34
Q

Putrecine

A

Ornithine

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35
Q

Arginine

A

Citrulline

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36
Q

LOA Test positive

A

purple

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37
Q

loa test negative

A

yellow

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38
Q

loa test medium

A

Moeller’s decarboxylase medium

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39
Q

loa test indicator

A

Bromcresol purple

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40
Q

To differentiate the different bacteria under
Enterobacteriaceae family according to their ability to
ferment different sugars

A

triple sugar ion

41
Q

TSI medium

A

TSI Agar (Butt-slant Medium)

42
Q

TSI pH indicator

A

Phenol Red

43
Q

phenol red color in acidic environment

A

yellow

44
Q

Ferrous sulfates + H2S

A

Black / Black precipitate

45
Q

A/A = 2-3 sugars fermented - ?

A

lactose fermenters

46
Q

? = glucose fermented – non lactose fermenters

A

K/A

47
Q

K/K = ? – non fermenter organisms

A

no sugar fermented

48
Q

Glucose + Lactose + Sucrose + Iron

A

KLIGLER’S IRON AGAR (KIA)

49
Q

Glucose + Lactose

A

RUSSELL’S DOUBLE AGAR (RDA)

50
Q

T/F: In TSI, glucose is seen in the butt of the medium

A

True

51
Q

T/F: In TSI, lactose and sucrose is seen in the butt of the medium

A

False

52
Q

lysine iron agar mediym

A

Moeller’s Medium

53
Q

lysine decarboxylation positive result

A

Purple

54
Q

Lysine Deamination positive result

A

Red

55
Q

Indole from tryptophan due to tryptophanase

A

indole test

56
Q

indole test medium

A

SIM medium

57
Q

Confirmatory reagent in indole test

A

Kovac’s/Erlich’s reagent

58
Q

what does SIM stand for

A

Sulfide Indole Motility Medium

59
Q

positive result in indole test

A

ring formation

60
Q

Filter paper strips impregnated with pdiaminocinnamaldehyde reagent use for anaerobic bacte

A

RAPID SPOT INDOLE TEST

61
Q

opposite of Voges-Proskauer

A

Methyl Red test

62
Q

principle of methyl red test

A

Mixed Acid Fermentation

63
Q

indicator of methyl red test

A

Methyl red

64
Q

Medium of methyl red test

A

MRVP medium

65
Q

MRVP medium is also known as

A

Clark and Lubs medium

66
Q

indole test positive color

A

RED

67
Q

indole test positive organism

A

E. cloaceae

68
Q

indole test negative color

A

yellow

69
Q

indole test negtaive organism

A

E. coli

70
Q

pH of E. coli

A

alkaline

71
Q

Butylene Glycol of Glucose Fermentation

A

Voges-Proskauer

72
Q

Voges-Proskauer also known as

A

Acetoin / Acetylmethylcarbinol

73
Q

Voges-Proskauer confimatory reagents

A

Alpha naphthol
40% KOH for enteric
Alpha naphthol in 40% KOH in Creatine

74
Q

40% KOH for Enteric

A

Barrits method

75
Q

Alpha naphthol in 40% KOH in creatine

A

Coblentz method

76
Q

voges-proskauer positive result color

A

red

77
Q

voges-proskauer positive result organism (KESH)

A

Kllebsiella, Enterobacter, Serratia, Hafnia

78
Q

voges-proskauer negative result color

A

yellow

79
Q

organism positive in Barrit’s method

A

E. cloacae

80
Q

organism negative in Barrit’s method

A

e. coli

81
Q

organism positive in Coblentz method

A

Streptococcus mutans

82
Q

Principle: Utilize citrate as source of carbon and energy

A

Citrate utilization

83
Q

Citrate utilzation medium

A

Simmon’s citrate

84
Q

Citrate utilzation indicator

A

bromthymol blue

85
Q

bromthymol blue in acidic

A

green

86
Q

bromthymol blue in alkaline

A

blue

87
Q

end product of citrate utilzation

A

Sodium carbonate

88
Q

citrate utilization positive organism

A

P. aeruginosa

89
Q

end product: Ammonium carbonate

A

UREA HYDROLYSIS TEST (UREASE)

90
Q

Urea hydrolysis test medium

A

Christensens agar

91
Q

Urea hydrolysis test indicator

A

phenol red

92
Q

Urea hydrolysis test positive color

A

RED/PINK

93
Q

identification for vibrio cholera

A

String test

94
Q

Medium of string test

A

0.5% sodium deoxycholate

95
Q

Esculin HOH test positive result color

A

black

96
Q

Esculin HOH test negative result color

A

yellow

97
Q

Gelatin hydrolysis test positive result

A

gel liquefies

98
Q

enzyme that destroys gelatin

A

gelatinase

BACTE LECTURE (3 decks)

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