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BACTE LECTURE > WEEK 7 > Flashcards

WEEK 7 Flashcards

1
Q

appearance of staphylococcus

A

gram positive cocci in cluster

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2
Q

appearance of micrococcus

A

gram positive cocci in tetrads/sarcinas

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3
Q

composition of BAP

A

nutrient agar + 5% sheep RBC

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4
Q

appearance of staphylococcus in BAP

A

creamy white colonies / pin head / beta hemolytic

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5
Q

incomplete hemolytic

A

alpha hemolytic

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6
Q

hemolytic pattern surrounded by green or brown zone

A

alpha hemolytic

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7
Q

complete hemolysis

A

beta hemolytic

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8
Q

colony is surrounded by clear zone

A

beta hemolytic

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9
Q

colony surrounded by inner alpha one of hemolysis and an outer b-zone of hemolysis

A

alpha prime

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10
Q

no hemolytic pattern

A

gamma hemolytic

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11
Q

main purpose is to enhance pigmentation of staphylococcus

A

Loeffler’s serum slant

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12
Q

Golden yellow in LSS

A

S. aureus

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13
Q

Lemon yellow in LSS

A

S. citreus

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14
Q

Porcelain white in LSS

A

S. albus / S. epidermidis

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15
Q

selective for micrococcaceae and differential for staphylococcus spp

A

Mannitol Salt Agar

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16
Q

inhibitor of MSA

A

7.5% NaCl

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17
Q

pH indicator of MSA

A

phenol red

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18
Q

(pH) appears yellow in MSA

A

Acidic

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19
Q

(pH) appears red to pink in MSA

A

Alkaline

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20
Q

carbohydrate component that differentiates mannitol fermenters organism with the use of pH indicator

A

mannitol

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21
Q

T/F: S. aureus is a mannitol fermenter

A

True

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22
Q

T/F: S. epidermidis is a mannitol fermenter

A

false

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23
Q

T/F S. saphrophyticus cannot ferment mannitol

A

true

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24
Q

test that differentiate staphylococcus from streptococcus

A

catalase test

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25
Q

T/F: Staphylococcus is catalase negative

A

false

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26
Q

T/F: Streptococcus is catalase negative

A

true

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27
Q

In catalase test, colony must be from ________

A

MSA plate

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28
Q

positive in anaerobic growth, resistant in bacitracin, facultative anaerobe, fermenter

A

staphylococcus

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29
Q

resistant in lysostaphin, negative in anaerobic growth, susceptible in bacitracin, oxidizer

A

micrococcus

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30
Q

pH indicator in Glucose Utilization Test

A

Bromthymol blue

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31
Q

Positive result in OF test

A

yellow

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32
Q

color of Alkaline in OF test

A

blue

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33
Q

Staphylococcus/Micrococcaceae: Ferments glucose

A

Staphylococcus

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34
Q

principle is converts fibrinogen to fibrin clot

A

coagulase test

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35
Q

detect cell bound coagulase

A

slide coagulase test

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36
Q

detects free coagulase

A

tube coagulase test

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37
Q

positive result in slide coagulase test

A

clumping

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38
Q

end product of citrate utilizer organism

A

calcium

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39
Q

positive result in tube coagulase test

A

gel-like clot

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40
Q

test for acetoin

A

VP test

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41
Q

Staphylococcus spp. VP test positive

A

S. aureus / S. schleiferi

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42
Q

Staphylococcus spp. PYRase test positive

A

S. intermedius / S. schleiferi

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43
Q

VP test negative, PYRase test negative

A

S. hyicus

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44
Q

Medium in DNAse test

A

DNA medium with methyl green

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45
Q

positive result in DNAse test

A

clearing of the medium

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46
Q

(3) organism DNAse positive

A

S. aureus
Moraxella
Serratia

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47
Q

test to differentiate Coagulase Negative Staphylococcus

A

Novobiocin Disc Test

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48
Q

Sensitive to novobiocin

A

S. epidermidis

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49
Q

Novobiocin resistant

A

S. saprophyticus

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50
Q

pathogenic determinants that will kill the WBC (anti-phagocytic)

A

Leukocidin / Panton-Valentin Factor

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51
Q
  • Ritter-Lyell Disease
  • Desquamation of palms and soles
  • Large flaccid bullae
A

exfoliatin

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52
Q

toxin responsible for food poisoning

A

Toxin A and B

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53
Q

Toxic shock syndrome toxin

A

F

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54
Q

responsible for the classification of streptococcaeae

A

M protein type

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55
Q

2 classification of streptococcaceae

A
  1. Lancefield classification

2. Brown & Smith classification

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56
Q

(Classification of Strepto) - CHO component of cell wall

A

lancefield classification

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57
Q

classification of strepto based on hemolytic pattern

A

brown and smith classification

58
Q

2 organism in Group A streptococcus

A

S. aureus

S. pyogenes

59
Q

golden crust lesions

A

impetigo

60
Q

bollous form

A

S. aureus

61
Q

thick crust

A

S. pyogenes

62
Q

Group A strep left untreated may cause:

A

Acute glomerulonephritis and Rheumatic fever

63
Q

common cause of neonatal meningitis

A

S. agalactiae

64
Q

Group B streptococcus organism

A

S. agalactiae

65
Q

Group C streptococcus organism

A

S. dysagalactiae

66
Q

Group D Streptococcus resistant to penicillin

A

Group D enterococcus

67
Q

2 organism Group D non-enterococcus

A

S. bovis

S. equinus

68
Q

cause of greenish brown discoloration

A

biliverdin

69
Q

alpha hemolytic, does not belong to lancefield classification

A

viridan streptococcus

70
Q

group A, beta hemolytic

A

S. pyogenes

71
Q

Group A produce 2 hemolysins which are:

A
  1. Streptolysin O

2. Streptolysin S

72
Q

Hemolysin: O2 labile

A

Streptolysin O

73
Q

Hemolysin: O2 stable

A

Streptolysin S

74
Q

Hemolysin: antigenic

A

Streptolysin O

75
Q

Hemolysin non antigenic

A

Streptolysin S

76
Q

Responsible for subsurface hemolysis

A

Streptolysin O

77
Q

Responsible for surface hemolysis

A

Streptolysin S

78
Q

Appearance of S. pneumoniae in gram stain

A

lancet/bullet/flame shaped diplococci

79
Q

appearance of streptococcus in BAP

A

white, pinpoint colonies

80
Q

reagent in Catalase test

A

3% H2O2

81
Q

detects if an organism can grown on 40% bile and can hydrolyze esculin

A

BEH test

82
Q

positive result in BEH test

A

Brown-Black precipitate

83
Q

Group of organism that is BEH test positive

A

Group D enterococci

84
Q

BEH test positive organism

A

Enterococcus fecalis

85
Q

Aside from Group D Enterococci _______ also grows on 6.5% NaCl

A

Group B

86
Q

Aside from Group D enterocococci, Group A also positive on _______ test

A

PYRase

87
Q

test to differentiare beta hemolytic streptoccocus

A

PYRase test

88
Q

PYRase enzyme

A

Pyrolidonylarylamidase

89
Q

substrate in PYRase test

A

l-pyrolidonyl-beta-naphthylamide

90
Q

indicator in PYRase test

A

P=dimethylaminocinnamaldehyde

91
Q

positive test in PYRase test

A

red color formation

92
Q

BEH positive, PYRase test positive

A

Group D enterococci

93
Q

BEH negative, PYRase test positive

A

Group A

94
Q

factors that enhances zone of hemolysis

A

CAMP factor

95
Q

positive result in CAMP

A

arrow zone of hemolysis

96
Q

the only streptococcus spp that is CAMP positive

A

S. agalactiae

97
Q

Catalse positive, CAMP positive

A

Listeria monocytogenes

98
Q

organism positive in Reverse camp test

A

Clostridium perfringens

99
Q

enzyme that degrade Na hippurate to Benzoic acid and glycine

A

hippuricase

100
Q

detects if an organism can hydrolyze Na hippurate to Benzoic acid and glycine

A

hippuric hydrolysis test

101
Q

positive result in hippuric hydrolysis test

A

purple

102
Q

units of bacitracin in SXT

A

0.04 units

103
Q

3 groups that are resistant to both taxo a and sxt

A

group b
group d entero
groupd d non entero

104
Q

was name after group a exhibit sensitivity to bacitracin

A

taxo A

105
Q

most preferred test for Group A

A

PYRase resr

106
Q

scarlet fever susceptibility test

A

Dick’s test

107
Q

scarlet fever diagnostic test

A

Schultz-Charlton

108
Q

arm + erythrogenic toxin

A

dick’s test

109
Q

Arm (rashes) + Antitoxin

A

Schultz-Charlton

110
Q

positive in dick’s test

A

redness or eythema

111
Q

positive in Schultz-Charlton

A

Neutralization or Blanching phenomena (pale)

112
Q

associated with lobar ppneumonia

A

Streptococcus pneumoniae

113
Q

sub-acute bacterial endocarditis

A

Viridans streptococcus

114
Q

associated with Dental Carries

A

Streptococcus mutans

115
Q

detects pneumococcal Antibody

A

Francis test

116
Q

ethylhyrocupreine Hydrochloride

A

Optochin

117
Q

requirements in NVS

A

Cysteine and Pyridoxal (Vit B6)

118
Q

organisms that do not grow in BAP

A

abiotrophia

119
Q

2 streptococcus spp that do not grow in BAP

A

Streptococcus adjacens

Streptococcus defectivus

120
Q

neisseria that is not kidney bean shaped

A

Neisseria elongata (Rod)

121
Q

3 motile neisseria spp.

A

N. flava,
N.subflava,
V. flavescens

122
Q

reagent in oxidase test

A

tetramethyl paraphenylene diamine dihydrochloride.

123
Q

oxidase positive result (color)

A

dark purple

124
Q

common cause of meningitis

A

N. meningitis

125
Q

bleeding or hemorrhages in ADRENAL GLAND

A

Waterhouse-Friderichsen syndrome

126
Q

associated with gonorrhoea/CLAP/STD

A

Neisseria gonorrheae

127
Q

modern treatment for opthalmia neonatorum

A

penicillin

128
Q

Test for presumptive Identification of Neisseria gonorrhoeae

A

SUPEROXOL TEST

129
Q

reagent in Test for presumptive Identification of Neisseria gonorrhoeae

A

30% H2O2

130
Q

positive result in Test for presumptive Identification of Neisseria gonorrhoeae

A

bubble formation

131
Q

Ab that inhibits gram positive bacteria

A

vancomysin

132
Q

Ab that inhibits gram negative bacteria except neisseria

A

colistin

133
Q

inhibits anti-fungal

A

nystatin

134
Q

Culture: Enriched Cap with antibiotics (4 culture)

A

TM
MTM
NYCA
ML

135
Q

Fermentation of CHO

A

CTA medium( Cysteine Trypticase agar)

136
Q

Neisseria positive for glucose and maltose only

A

N. meningitidis

137
Q

Neisseria positive for glucose only

A

N. gonorrhoeae

138
Q

positive for glucose, maltose, lactose and negative for sucrose

A

N. lactamica

139
Q

glucose, maltose, and sucrose positive but cannot ferment lactose

A

N. sicca

140
Q

colonies look like hockey puck

A

moraxella catarrhalis

141
Q

asaccharolytic

A

Moraxella

BACTE LECTURE (3 decks)

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