WEEK 7 Flashcards
1
Q
appearance of staphylococcus
A
gram positive cocci in cluster
2
Q
appearance of micrococcus
A
gram positive cocci in tetrads/sarcinas
3
Q
composition of BAP
A
nutrient agar + 5% sheep RBC
4
Q
appearance of staphylococcus in BAP
A
creamy white colonies / pin head / beta hemolytic
5
Q
incomplete hemolytic
A
alpha hemolytic
6
Q
hemolytic pattern surrounded by green or brown zone
A
alpha hemolytic
7
Q
complete hemolysis
A
beta hemolytic
8
Q
colony is surrounded by clear zone
A
beta hemolytic
9
Q
colony surrounded by inner alpha one of hemolysis and an outer b-zone of hemolysis
A
alpha prime
10
Q
no hemolytic pattern
A
gamma hemolytic
11
Q
main purpose is to enhance pigmentation of staphylococcus
A
Loeffler’s serum slant
12
Q
Golden yellow in LSS
A
S. aureus
13
Q
Lemon yellow in LSS
A
S. citreus
14
Q
Porcelain white in LSS
A
S. albus / S. epidermidis
15
Q
selective for micrococcaceae and differential for staphylococcus spp
A
Mannitol Salt Agar
16
Q
inhibitor of MSA
A
7.5% NaCl
17
Q
pH indicator of MSA
A
phenol red
18
Q
(pH) appears yellow in MSA
A
Acidic
19
Q
(pH) appears red to pink in MSA
A
Alkaline
20
Q
carbohydrate component that differentiates mannitol fermenters organism with the use of pH indicator
A
mannitol
21
Q
T/F: S. aureus is a mannitol fermenter
A
True
22
Q
T/F: S. epidermidis is a mannitol fermenter
A
false
23
Q
T/F S. saphrophyticus cannot ferment mannitol
A
true
24
Q
test that differentiate staphylococcus from streptococcus
A
catalase test
25
T/F: Staphylococcus is catalase negative
false
26
T/F: Streptococcus is catalase negative
true
27
In catalase test, colony must be from ________
MSA plate
28
positive in anaerobic growth, resistant in bacitracin, facultative anaerobe, fermenter
staphylococcus
29
resistant in lysostaphin, negative in anaerobic growth, susceptible in bacitracin, oxidizer
micrococcus
30
pH indicator in Glucose Utilization Test
Bromthymol blue
31
Positive result in OF test
yellow
32
color of Alkaline in OF test
blue
33
Staphylococcus/Micrococcaceae: Ferments glucose
Staphylococcus
34
principle is converts fibrinogen to fibrin clot
coagulase test
35
detect cell bound coagulase
slide coagulase test
36
detects free coagulase
tube coagulase test
37
positive result in slide coagulase test
clumping
38
end product of citrate utilizer organism
calcium
39
positive result in tube coagulase test
gel-like clot
40
test for acetoin
VP test
41
Staphylococcus spp. VP test positive
S. aureus / S. schleiferi
42
Staphylococcus spp. PYRase test positive
S. intermedius / S. schleiferi
43
VP test negative, PYRase test negative
S. hyicus
44
Medium in DNAse test
DNA medium with methyl green
45
positive result in DNAse test
clearing of the medium
46
(3) organism DNAse positive
S. aureus
Moraxella
Serratia
47
test to differentiate Coagulase Negative Staphylococcus
Novobiocin Disc Test
48
Sensitive to novobiocin
S. epidermidis
49
Novobiocin resistant
S. saprophyticus
50
pathogenic determinants that will kill the WBC (anti-phagocytic)
Leukocidin / Panton-Valentin Factor
51
- Ritter-Lyell Disease
- Desquamation of palms and soles
- Large flaccid bullae
exfoliatin
52
toxin responsible for food poisoning
Toxin A and B
53
Toxic shock syndrome toxin
F
54
responsible for the classification of streptococcaeae
M protein type
55
2 classification of streptococcaceae
1. Lancefield classification
| 2. Brown & Smith classification
56
(Classification of Strepto) - CHO component of cell wall
lancefield classification
57
classification of strepto based on hemolytic pattern
brown and smith classification
58
2 organism in Group A streptococcus
S. aureus
| S. pyogenes
59
golden crust lesions
impetigo
60
bollous form
S. aureus
61
thick crust
S. pyogenes
62
Group A strep left untreated may cause:
Acute glomerulonephritis and Rheumatic fever
63
common cause of neonatal meningitis
S. agalactiae
64
Group B streptococcus organism
S. agalactiae
65
Group C streptococcus organism
S. dysagalactiae
66
Group D Streptococcus resistant to penicillin
Group D enterococcus
67
2 organism Group D non-enterococcus
S. bovis
| S. equinus
68
cause of greenish brown discoloration
biliverdin
69
alpha hemolytic, does not belong to lancefield classification
viridan streptococcus
70
group A, beta hemolytic
S. pyogenes
71
Group A produce 2 hemolysins which are:
1. Streptolysin O
| 2. Streptolysin S
72
Hemolysin: O2 labile
Streptolysin O
73
Hemolysin: O2 stable
Streptolysin S
74
Hemolysin: antigenic
Streptolysin O
75
Hemolysin non antigenic
Streptolysin S
76
Responsible for subsurface hemolysis
Streptolysin O
77
Responsible for surface hemolysis
Streptolysin S
78
Appearance of S. pneumoniae in gram stain
lancet/bullet/flame shaped diplococci
79
appearance of streptococcus in BAP
white, pinpoint colonies
80
reagent in Catalase test
3% H2O2
81
detects if an organism can grown on 40% bile and can hydrolyze esculin
BEH test
82
positive result in BEH test
Brown-Black precipitate
83
Group of organism that is BEH test positive
Group D enterococci
84
BEH test positive organism
Enterococcus fecalis
85
Aside from Group D Enterococci _______ also grows on 6.5% NaCl
Group B
86
Aside from Group D enterocococci, Group A also positive on _______ test
PYRase
87
test to differentiare beta hemolytic streptoccocus
PYRase test
88
PYRase enzyme
Pyrolidonylarylamidase
89
substrate in PYRase test
l-pyrolidonyl-beta-naphthylamide
90
indicator in PYRase test
P=dimethylaminocinnamaldehyde
91
positive test in PYRase test
red color formation
92
BEH positive, PYRase test positive
Group D enterococci
93
BEH negative, PYRase test positive
Group A
94
factors that enhances zone of hemolysis
CAMP factor
95
positive result in CAMP
arrow zone of hemolysis
96
the only streptococcus spp that is CAMP positive
S. agalactiae
97
Catalse positive, CAMP positive
Listeria monocytogenes
98
organism positive in Reverse camp test
Clostridium perfringens
99
enzyme that degrade Na hippurate to Benzoic acid and glycine
hippuricase
100
detects if an organism can hydrolyze Na hippurate to Benzoic acid and glycine
hippuric hydrolysis test
101
positive result in hippuric hydrolysis test
purple
102
units of bacitracin in SXT
0.04 units
103
3 groups that are resistant to both taxo a and sxt
group b
group d entero
groupd d non entero
104
was name after group a exhibit sensitivity to bacitracin
taxo A
105
most preferred test for Group A
PYRase resr
106
scarlet fever susceptibility test
Dick's test
107
scarlet fever diagnostic test
Schultz-Charlton
108
arm + erythrogenic toxin
dick's test
109
Arm (rashes) + Antitoxin
Schultz-Charlton
110
positive in dick's test
redness or eythema
111
positive in Schultz-Charlton
Neutralization or Blanching phenomena (pale)
112
associated with lobar ppneumonia
Streptococcus pneumoniae
113
sub-acute bacterial endocarditis
Viridans streptococcus
114
associated with Dental Carries
Streptococcus mutans
115
detects pneumococcal Antibody
Francis test
116
ethylhyrocupreine Hydrochloride
Optochin
117
requirements in NVS
Cysteine and Pyridoxal (Vit B6)
118
organisms that do not grow in BAP
abiotrophia
119
2 streptococcus spp that do not grow in BAP
Streptococcus adjacens
| Streptococcus defectivus
120
neisseria that is not kidney bean shaped
Neisseria elongata (Rod)
121
3 motile neisseria spp.
N. flava,
N.subflava,
V. flavescens
122
reagent in oxidase test
tetramethyl paraphenylene diamine dihydrochloride.
123
oxidase positive result (color)
dark purple
124
common cause of meningitis
N. meningitis
125
bleeding or hemorrhages in ADRENAL GLAND
Waterhouse-Friderichsen syndrome
126
associated with gonorrhoea/CLAP/STD
Neisseria gonorrheae
127
modern treatment for opthalmia neonatorum
penicillin
128
Test for presumptive Identification of Neisseria gonorrhoeae
SUPEROXOL TEST
129
reagent in Test for presumptive Identification of Neisseria gonorrhoeae
30% H2O2
130
positive result in Test for presumptive Identification of Neisseria gonorrhoeae
bubble formation
131
Ab that inhibits gram positive bacteria
vancomysin
132
Ab that inhibits gram negative bacteria except neisseria
colistin
133
inhibits anti-fungal
nystatin
134
Culture: Enriched Cap with antibiotics (4 culture)
TM
MTM
NYCA
ML
135
Fermentation of CHO
CTA medium( Cysteine Trypticase agar)
136
Neisseria positive for glucose and maltose only
N. meningitidis
137
Neisseria positive for glucose only
N. gonorrhoeae
138
positive for glucose, maltose, lactose and negative for sucrose
N. lactamica
139
glucose, maltose, and sucrose positive but cannot ferment lactose
N. sicca
140
colonies look like hockey puck
moraxella catarrhalis
141
asaccharolytic
Moraxella
