Week 7 - pharmacology and patient safety Flashcards
Explain how hormones are selective in which cells they interact with and affect. (LO1)
- Hormones only affect cells that possess the necessary receptors.
- Receptors for a specific hormone may be found on many different cells or may be limited to a small number of specialised cells.
- E.g. thyroid hormones act on many different tissue types, stimulating metabolic activity throughout the body.
- Cells also possess receptors for different types of hormones.
- The sensitivity and response of a cell to a particular hormone is determined by the number of receptors for the hormone.
- The number of receptors that respond to a hormone can change over time, resulting in a fluctuation of sensitivity.
What is meant by upregulation with regards to hormone sensitivity? (LO1)
- Usually in response to rising hormone levels.
- The number of receptors to the hormone on the cell surface increases.
- Cell has increased sensitivity to the hormone.
- Increased cellular activity.
What is meant by downregulation with regards to hormone sensitivity? (LO1)
- Usually in response to rising hormone levels.
- The number of receptors to the hormone on the cell surface decreases.
- Cell has decreased sensitivity to the hormone.
- Reduced cellular activity.
What are direct-acting hormones? Give some examples. (LO1)
- Lipid-derived (soluble) hormones.
Examples:
- Steroid hormones.
- Vitamin D.
- Thyroxine.
How do direct-acting hormones work? (LO1)
- The hormone diffuses into the cytoplasm and either:
A) binds to intracellular receptor and the hormone receptor complex relocates to the nucleus (e.g. glucocorticoids).
B) or, relocates to the nucleus and then binds to the receptor in the nucleus (e.g. oestrogen, androgens, thyroid hormones). - The hormone receptor complex binds to hormone response elements on the DNA.
- The hormones and receptor complex act as transcription regulators by increasing or decreasing the synthesis of mRNA molecules of specific genes.
- This, in turn, determines the amount of corresponding protein that is synthesised by altering gene expression.
What are indirect-acting hormones? (LO1)
- Non-lipid soluble hormones (amino acid or polypeptide-derived).
- These cannot directly act upon DNA.
List some classes of receptors that indirect-acting hormones interact with. (LO1)
- G-protein coupled receptors (GPCR).
- Receptor tyrosine kinases (RTK).
- Guanylyl cyclase receptors.
Describe the basic structure of a G-protein coupled receptor (GPCR). (LO1)
- Made up of 3 subunits: α, β and γ.
- When no hormone is bound, the GPCR is inactive and the α-subunit is bound to guanosine diphosphate (GDP).
- When a hormone binds to the receptor, the receptor conformation changes, allowing guanosine triphosphate (GTP) to bind.
- After binding, GTP is hydrolysed by the GPCR into GDP and becomes inactive.
- The α-subunit will then dissociate and bind to another effector protein (cAMP, calcium ion channel or PLC).
- GPCRs are named according to the alpha subunit: Gₛ, Gᵢ, Gq.
Describe the function of the Gs subtype of GPCR. Give some examples. (LO1)
- Stimulates adenylate cyclase.
- This increases cAMP.
- Leads to activation of protein kinase A.
Examples:
- Beta-1 receptors - adrenaline, noradrenaline.
- Beta-2 receptors - adrenaline, salbutamol.
- H2 receptors - histamine.
- D1 receptors - dopamine.
- V2 receptors - vasopressin.
- Receptors for ACTH, LH, FSH, PTH, glucagon, calcitonin and prostaglandins.
Describe the function of the Gi subtype of GPCR. Give some examples. (LO1)
- Inhibites adenylate cyclase.
- This decreases cAMP.
- Leads to inhibition of protein kinase A.
Examples:
- M2 receptors - acetylcholine.
- Alpha-2 receptors - adrenaline, noradrenaline.
- D2 receptors - dopamine.
- GABA-B receptor.
Describe the function of the Gq subtype of GPCR. Give some examples. (LO1)
- Activates phospholipase C.
- Splits PIP₂ into IP₃ and DAG.
- Leads to activation of protein kinase C.
Examples:
- Alpha-1 receptors - adrenaline, noradrenaline.
- H1 receptors - histamine.
- V1 receptors - vasopressin.
- M1, M3 receptors - acetylcholine.
Describe GPCR α-subunit interactions with cAMP. (LO1)
- The α-subunit of the activated G-protein dissociates, binds to and activates a membrane-bound enzyme called adenylyl cyclase.
- Adenylyl cyclase catalyses the conversion of ATP to cAMP.
- cAMP activates protein kinases.
- Protein kinases transfer a phosphate group from ATP to a substrate molecule during phosphorylation.
- The phosphorylation of a substrate molecule changes its structural orientation, thereby activating it.
- These activated molecules can mediate changes in cellular processes.
Describe how the effect of a hormone is amplified when a GPCR α-subunit interacts with cAMP. (LO1)
- As the signalling process progresses, the hormone’s effects are amplified.
- The binding of the hormone at a single receptor causes the activation of MANY GPCRs which activates adenylyl cyclase.
- Each molecule of adenylyl cyclase then triggers the formation of MANY cAMP molecules.
- Once activated by cAMP, protein kinases can catalyse MANY reactions.
In this way, a small amount of hormone can trigger the formation of a large amount of cellular product.
Describe the role of phosphodiesterase (PDE). (LO1)
- When a GPCR α-subunit interacts with cAMP due to the presence of a hormone, an amplification process is started.
- To stop hormone activity, cAMP is deactivated by the cytoplasmic enzyme phosphodiesterase (PDE).
- PDE is always present in the cell and breaks down cAMP to control hormone activity, preventing overproduction of cellular production - e.g. glucagon.
Describe GPCR α-subunit interactions with phospholipase C (PLC). (LO1)
- The α-subunit binds to PLC.
- This converts PIP₂ into IP₃ and diacylglycerol (DAG).
- IP₃ and DAG regulate the activity of enzymes.
- IP₃ releases calcium ions from the endoplasmic reticulum and opens calcium channels in the plasma membrane.
- DAG activates protein kinase C which phosphorylates other proteins and activates them - e.g. adrenaline.
Describe the function of IP₃. (LO1)
IP₃ releases calcium ions from the endoplasmic reticulum and opens calcium channels in the plasma membrane.
Describe the function of DAG. (LO1)
DAG activates protein kinase C which phosphorylates other proteins and activates them - e.g. adrenaline.
Describe GPCR α-subunit interactions with calcium channels. (LO1)
- Here, the calcium channels act as molecular switches inside the cell - e.g. adrenaline.
- The α-subunit binds to a calcium channel, causing it to open.
- Calcium ions move into the cell, leading to an increase in intracellular mediators.
- When the α-subunit hydrolyses the attached GTP to GDP, the α-subunit will dissociate from the calcium channel, closing it so no more calcium ions move into the cell.
Describe the function of receptor tyrose kinases (RTK). (LO1)
- These are enzyme-linked receptors.
- RTKs phosphorylate tyrosine to allow signal transmission to other parts of the cell.
- The phosphorylated receptors act as a docking platform for their proteins that contain special types of binding domains.
- RTKs play an important role in growth factors, signalling molecules that promote cell division and survival - e.g. platelet-derived growth factor (PDGF), nerve growth factor (NGF), insulin, epidermal growth factor.
Describe the function of guanylyl cyclase receptors. (LO1)
- These contain intrinsic enzyme activity.
- The hormone binds to the receptor linked to guanylyl cyclase (GC), causing a conformational change of the receptor.
- This leads to the conversion of guanosine triphosphate (GTP) into cytoguanosine monophosphate (cGMP).
- The cGMP activates protein kinases and these then phosphorylate other proteins to activate them.
- E.g. atrial natriuretic factor, brain natriuretic factor.
Define what is meant by lipids. (LO2)
- This is collective name for all fats and fat-like substances.
- These are water-insoluble but dissolve in organic solvents like alcohol.
List the five types of lipids. (LO2)
- Fatty acids.
- Triglycerides (fats and oils).
- Glycerophospholipids (membrane lipids).
- Sphingolipids (membrane lipids).
- Cholesterol.
Describe fatty acids. (LO2)
- Between 14-24 carbon atoms.
- Most common have 16-18 carbon atoms.
- Unsaturated fatty acids contain double bonds.
- Most unsaturated fatty acids are in cis-configuration.
- In cis, the functional groups are on the same side.
- In trans, the functional groups are on opposite sides.
- Fatty acids are described by the number of carbon atoms and the number of double bonds in them.
- E.g. Palmitic acid is C16:0 - this means 16 carbon atoms and 0 double bonds.
- Most fatty acids have an even number of carbon atoms.
Explain how the melting points of fatty acids vary. (LO2)
- Saturated fatty acids have a higher melting point and it increases with the length of the molecule.
- Unsaturated fatty acids have lower melting points than saturated fatty acids of the same length.
- For unsaturated fatty acids of the same length, the melting point decreases with the number of double bonds.
What is the difference between saturated and unsaturated fatty acids? (LO2)
- Saturated fatty acids don’t have any double bonds.
- Unsaturated fatty acids have double bonds.
To make it easier to remember:
When double bonds are introduced, there are fewer hydrogen atoms.
Saturated basically means that the molecule is saturated with hydrogen atoms so it must not have any double bonds.
Why do saturated fatty acids have a higher melting point than unsaturated fatty acids? (LO2)
They have a higher melting point because the molecule has the ability to closely pack with other molecules of this nature (due to the lack of double bonds) and forms intermolecular interactions which require a lot of energy to break.
What is linoleic acid? (LO2)
- Vitamin F.
- An essential fatty acid that the human body cannot synthesise.
- Must be taken up with food.
What is arachidonic acid? (LO2)
- Can be synthesised from linoleic acid so it’s not an essential fatty acid.
How can omega-6 be obtained from our diet? (LO2)
Omega-6 is present in:
- Vegetable oil.
- Seeds.
- Soy bean.
- Canola oil.
- Fish oil - fatty fish like salmon and sardines.
What are prostaglandins, thromboxanes and leukotrienes? (LO2)
- These are all inflammatory mediators.
- Their precursor is fatty acids.
- Prostaglandins are technically hormones and have a variety of functions in the body.
- Leukotrienes are powerful constrictor of bronchial and intestinal smooth muscle. They also increase the permeability of capillaries.
How are prostaglandins and leukotrienes produced from fatty acids? (LO2)
- Linoleic acid is converted to eicosatreinoic acid.
- Eicosatreinoic acid is the precursor for prostaglandins F1 and F1-α (PGF₁ and PGF₁α).
- Eicosatreinoic acid is further converted to arachidonic acid which is the precursor for leukotrienes and other prostaglandins - e.g. PGE₂α, PGF₂, PGD₂, PGI₂ (prostacyclin), TXA₂.
- Alternatively, linoleic acid is converted to eicosapentaenoic acid which is the precursor for prostaglandins, PGE₃ and PGF₃α.
Describe the transport and storage of triglycerides. (LO2)
- Triglycerides are not absorbed as whole molecules by adipocytes. They are broken up before they’re taken up.
- Triglycerides from the liver are transported by very low density lipoproteins (VLDL) to the adipocytes. Lipoprotein lipase splits triglycerides to fatty acids and glycerol.
- Fatty acids are taken up into the fat cells and activated to form acyl-CoA.
- The glycerol comes from glucose so glucose is taken up by the fat cells and metabolised to glycerol-3-phosphate.
- Glycerol-3-phosphate and 3 molecules of acyl-CoA join together to form one molecule of triglyceride.
- In the event that the body needs energy, stored triglycerides are remobilised. A hormone-sensitive lipase enzyme splits the triglyceride into glycerol and fatty acids.
- The fatty acids are released into the blood and are transported around the body in the form of fatty acid-albumin complex.
Describe the structure of glycerophospholipids. (LO2)
- In glycerophospholipids, the central glycerol molecule is linked to two fatty acid molecules and via a phosphate group to an alcohol.
- Main components of cell membranes.
- They are amphiphilic - have hydrophilic and hydrophobic parts.
- The fatty acids are the hydrophic parts.
- The phosphate and alcohol groups are the hydrophilic regions.
Describe the generic chemical structure of glycerophospholipids. (LO2)
- Two hydroxyl groups off the central glycerol are esterified with fatty acids.
- The third hydroxyl group is esterified by phosphoric acid which is further esterified by an alcohol.
If the alcohol in a glycerophospholipid is ethanolamine, what is the molecule known as? (LO2)
Phosphatidylethanolamine.
- Membranes contain around 13-35% of this.
If the alcohol in a glycerophospholipid is choline, what is the molecule known as? (LO2)
Phosphtidylcholine.
- A.k.a lecithin.
- Membranes contain around 39-58% of this.
If the alcohol in a glycerophospholipid is the amino acid, serine, what is the molecule known as? (LO2)
Phosphatidylserine.
- Membranes contain around 1-9% of this.
If the alcohol in a glycerophospholipid is the sugar, inositol, what is the molecule known as? (LO2)
Phosphatidylinositol.
- Membranes contain around 5-12% of this.
List the enzymes involved in the cleavage and resorption of glycerophospholipids and their function. (LO2)
- Phospholipase A₁ - cleaves off the fatty acid at position 1, producing 1-acyl-lisophospholipid.
- Phospholipase A₂ - cleaves off the fatty acid at position 2, producing 2-acyl-lisophospholipid.
- Phospholipase C - cleaves off the phosphorylated alcohol, producing 1,2-diacylglycerol.
- Phospholipase D - cleaves off the alcohol, producing phosphatidic acid.
- By the action of all four phospholipases, glycerophospholipases are broken down into glycerol, fatty acids, phosphates and alcohol.
Describe the cleavage and resorption of glycerophospholipids. (LO2)
- Resorption of dietary glycerophospholipids takes place in the digestive tract.
- Before glycerophospholipids can be resorbed, they need to be cleaved into their building blocks by phospholipases of the pancreas.
- By the action of all four phospholipases, glycerophospholipids are broken down into glycerol, fatty acids, phosphates and alcohol.
What are sphingolipids? (LO2)
- Components of cell membranes.
- There are amphiphilic (contain both hydrophobic and hydrophilic parts).
- There are two main sphingolipids:
1. Sphingomyelin.
2. Glycolipids.
Describe the structure of sphingomyelin. (LO2)
- The central long chain amino alcohol, sphingosine, is linked to one fatty acid molecule and two phosphocholine molecules.
- The fatty acid and part of the sphingosine are hydrophobic parts.
- Phosphocholine is hydrophilic.
Describe the structure of glycolipids. (LO2)
- The central sphingosine is linked to one fatty acid molecule and two sugars.
- The fatty acid and part of the sphingosine are hydrophobic parts.
- The sugars are hydrophilic.
Describe the generic chemical structure of sphingolipids. (LO2)
- The central sphingosine is an 18 carbon amino alcohol with an unsaturated hydrocarbon chain.
- The amine group is esterified to a fatty acid.
- The hydrocarbon chain and the fatty acid anchor the sphingosine molecule in lipid membranes.
If the hydroxyl group in a spingolipid is not esterified, what is the sphingolipid then known as? (LO2)
Ceramide.
- Important intermediate in the metabolism of sphingolipids.
If the hydroxyl group in a spingolipid is esterified to phosphorylcholine, what is the sphingolipid then known as? (LO2)
Sphingomyelin.
- They make up approximately 1-20% of membrane lipids.
If the hydroxyl group in a sphingolipid is esterified to a sugar, what is the sphingolipid then known as? (LO2)
Cerebroside.
- The most common cerebrosides are glucocerebrosides and galactocerebrosides.
What are gangliosides? What is their function? (LO2)
- These are the most complex sphingolipids.
- They contain oligosaccharide groups with one or more sialic acids (Glc-Gla-NANA).
- Gangliosides have many functions, including cell-cell recognition, as receptors for baterial toxins, e.g. cholera toxin, and as receptors for certain pituitary glycoprotein hormones.
Describe the degradation of sphingolipids. (LO2)
- The degradation of sphingolipids occurs in the lysosomes by exoglycosidase.
- Deficiency of any of these enzymes leads to an accumulation of substrate in the lysosome.
- Ganglioside M1 and globoside are both eventually converted into lactosyl ceramide as a final product.
List the enzymes involved in the degradation of sphingolipids and their function. (LO2)
Ganglioside degradation:
- GM1-β-galactosidase - converts ganglioside GM1 into ganglioside GM2.
- Hexosaminidase A - converts ganglioside GM2 into ganglioside GM3.
- Ganglioside neuraminidase converts ganglioside GM3 into lactosyl ceramide.
Globoside degradation:
- Hexosaminidase A+B - converts globoside into trihexosylceramide.
- α-galactosidase A - converts trihexosylceramide into lactosyl ceramide.
What is it known as when there is a defiency of the enzymes involved in the degradation of the sphingolipids? (LO2)
Resulting diseases are called lipid-storage diseases or sphingolipidoses. These are genetic.
What disease occurs when GM1-β-galactosidase is dysfunctional/deficient? What are the features of this disease? (LO2)
Note: lipid-storage disease.
GM1 gangliodosis.
Features:
- Mental retardation.
- Liver enlargement.
- Skeletal involvement.
- Death by age 2.
What disease occurs when hexosaminidase A is dysfunctional/deficient? What are the features of this disease?(LO2)
Note: lipid-storage disease.
Tay-Sachs disease.
Features:
- Mental retardation.
- Blindness.
- Death by age 3.
What disease occurs when hexosaminidase A and B are both dysfunctional/deficient? What are the features of this disease? (LO2)
Note: lipid-storage disease.
Sandhoff’s disease.
Features:
- Similar to Tay-Sachs but more rapidly progressing.
- Mental retardation.
- Blindness.
What disease occurs when α-galactosidase A is dysfunctional/deficient? What are the features of this disease? (LO2)
Note: lipid-storage disease.
Fabry’s disease.
Features:
- Skin rash.
- Kidney failure.
- Pain in lower extremities.
Why is no disease associated with the dysfunction/deficiency of ganglioside neuraminidase? (LO2)
Note: lipid-storage disease.
No genetic disorder is known for the dysfunction of ganglioside neuraminidase because this enzyme is so essential that any foetus with a deficiency of it wouldn’t survive.
List the enzymes involved in the degradation of lactosyl ceramide, sphingomyelin and sulfatide and their function. (LO2)
Lactosyl ceramide:
- β-galactosidase - converts lactosyl ceramide
- Glucocerebrosidase - converts glucocerebroside to ceramide.
Sphingomyelin:
1. Sphingomyelinase - converts sphingomyelin to ceramide.
Sulfatide:
- Arylsulfatase A - converts sulfatide to galactocerebroside.
- Galactocerebrosidase - converts galactocerebroside to ceramide.
All three products above are converted to ceramide which is further degraded to fatty acids:
1. Ceramidase - converts ceramide to fatty acids.
What disease occurs when glucocerebrosidase is dysfunctional/deficient? What are the features of this disease? (LO2)
Note: lipid-storage disease.
Gaucher’s disease.
Features:
- Liver and spleen enlargement.
- Erosion of long bones.
- Mental retardation in infantile form only.
What disease occurs when sphingomyelinase is dysfunctional/deficient? What are the features of this disease? (LO2)
Note: lipid-storage disease.
Neimann-Park disease.
Features:
- Liver and spleen enlargement.
- Mental retardation.
What disease occurs when arylsulfatase A is dysfunctional/deficient? What are the features of this disease? (LO2)
Note: lipid-storage disease.
Sulfatide lipidosis.
Features:
- Mental retardation.
- Death in first decade.
What disease occurs when galactocerebrosidase is dysfunctional/deficient? What are the features of this disease? (LO2)
Note: lipid-storage disease.
Krabbe’s disease.
Features:
- Loss of myelin.
- Mental retardation.
- Death by age 2.
What disease occurs when ceramidase is dysfunctional/deficient? What are the features of this disease? (LO2)
Note: lipid-storage disease.
Farber’s lipogranulomatosis.
Features:
- Painful and progressive deformed joints.
- Skin nodules.
- Death within a few years.
Why is no disease associated with the dysfunction/deficiency of β-galactosidase? (LO2)
Note: lipid-storage disease.
There is no genetic disorder known for when β-galactosidase is affected because this enzyme seems to be so essential that a foetus with a dysfunction/deficiency of it would not survive.
When these lipid degradation enzymes are dysfunctional, why does the accumulation of substrates cause lipid-storage diseases? (LO2)
- Because the unmetabolised sphingolipids accumulate to toxic levels that affects the function of nerve cells and other cells.
- The most common disease is Tay-Sachs disease - 1:300.
- There is no cure for lipid-storage diseases but Gaucher’s and Fabry’s disease can be treated with enzyme replacement therapy.
Describe the structure of cholesterol. (LO2)
- Consists of a condensed 4-ring system, also known as a steroid ring system.
- Almost the whole cholesterol molecule is hydrophobic.
- Only the hydroxyl group is the hydrophilic part of the molecule.
- This makes it weakly amphiphilic.
List the sources of cholesterol. (LO2)
Dietary intake:
- 300mg/day.
- Can vary with different diets.
Endogenous synthesis:
- 1000mg/day.
Where in the body is cholesterol mainly synthesised? (LO2)
All cells are capable of synthesising cholesterol but the main sites are the liver and intestines.
Cholesterol acts as a precursor for other molecules. List some of these. (LO2)
- Bile acids - glycocholate (most common bile salt).
- Vitamin D3.
- Glucocorticoids - cortisol, produced from cholesterol by the adrenocortex. Glucorticoids support gluconeogenesis and glycogen production.
- Mineralocorticoids - aldosterone, produced from cholesterol by the adrenocortex. Mineralocorticoids increase the resorption of sodium chloride and bicarbonate in the kidneys.
- Androgens - testosterone, by the testis.
- Progestogens - progesterone, by the yellow body of the corpus luteum.
- Oestrogens - estrone, by the ovaries.
Describe the metabolism of fatty acids. (LO2)
- Fatty acids can be broken down to acetyl-CoA by a process called β-oxidation.
- Acetyl-CoA is the building block for synthesis of cholesterol.
- Glucose can be broken down to acetyl-CoA via glycolysis which can then be used to synthesise fatty acids.
- Glucose can also be broken down to glycerol which, in combination with fatty acids, can form the triglycerides and glycerophospholipids.
- The fatty acid, palmitate, is a precursor for sphingosine, which alone with a second fatty acid, can form ceramides and sphingolipids.
What is nicotine? (LO3)
- A naturally occurring plant alkaloid.
- Found primarily in solanoceous plants - potato, tomato, green pepper, tobacco.
- Principle source is tobacco and replacement therapies, e.g. nicotine patch, nicotine gum.
- Structure: 1-methyl-2,3-pyridyl-pyrrolidine.
- Pure nicotine is a clear liquid with a characteristic odour but it turns brown on exposure to air.