Week 7 Flashcards
What is the endomembrane system in eukaryotes composed of?
Nuclear membrane, endoplasmic reticulum (RE, SE), golgi and lysosomes
What is the SER? (smooth endoplasmic reticulum)
Most cell types have relatively little smooth ER (SER)
Phospholipid, fat and steroid (including sex hormones) manufacturer
Carbohydrate metabolism
In hepatocytes, breaks down stored glycogen to release glucose
Detoxifies lipid-soluble drugs such as barbiturates
(Adds charged water-soluble groups such as sulphate or glycuronic acid)
What is a specialised form of the SER? What does it do?
Sarcoplasmic Reticulum (Found in muscle cells)
- Network of tubular sacs
- Transmits electrical signals
- Sequesters calcium ions from the cytosol
- The level of intercellular calcium regulates muscle contraction in muscle cells.
What do muscles consist of?
Myofibrils, surrounded by sarcoplasmic reticulum.
What are myofibrils made up of?
Repetitive arrangement of filaments called sarcomere. (The region where myosin and actin filaments overlap)
In summary, How do muscle contractions occur?
Muscle cells receive action potentials from the neuromuscular junction through T tubules in the sarcoplasmic reticulum.
The sarcoplasmic reticulum releases calcium ions.
Calcium ions bind to troponin causing a conformational change in tropomyosin.
Myosin and actin can now interact which results in muscle contraction.
What is myosin vs actin?
Myosin = thick filaments
Actin = Thin filaments
Briefly What is the sliding-filament model
Actin and myosin slide over each other during muscle contraction, as a result of an influx of Ca ions.
H line - shortens during contraction, only myosin
A line - region containing length of a single myosin filament
I like - contains length of single actin
Z line - Sarcomere boundary and junction of actin and myosin filaments
What are the 4 classes of protein synthesis? Where does it begin?
Secreted, glycosylated, lysosomal enzymes, membrane bound proteins.
Begins in the cytosol
What proteins attach to the RER during protein synthesis?
Only ribosomes synthesising proteins with a specific signal peptide sequence.
The N-terminus of these proteins contains a signal peptide usually 20-30 amino acids long
What is glycosylation (in the RER)?
The addition of sugars or oligosaccharides
Glycosylation in the RER?
An oligosaccharide added in the RER. Composed of N-acetylglucosamine, mannose and glucose residues containing a total of 14 sugar residues transferred to the proteins in the RER
What is glycosylation (in the golgi)?
The principle modification, which occurs to the proteins as they pass through the golgi
Glycosylation of proteins in the golgi is essential, why?
Essential to their function,
for example in the case of mucin produced these are glycoproteins with long highly oligosaccharide chains which are essential to produce a highly hydrated gel like material
What are lysosomes?
Vesicular structures that contain hydrolytic enzymes.
When a lysosome fuses with a target, H+ pumped into secondary lysosome to bring down pH, activate enzymes
Carry out autophagy (recycling of organelles), phagocytosis, and apoptosis (cell-suicide)
Where do primary lysosomes originate?
The trans face of the golgi
What causes a lysosomal storage disease?
Partially degraded insoluble metabolites accumulate within lysosomes if a particular lysosomal enzymes is defective.
Resulting material results in enlarged lysosomes that compromise cell function in over 50 different lysosomal storage diseases.
Example of a lysosomal storage disease?
Tay-Sachs disease
Haxosaminidase A enzyme deficiency results in the accumulation of lipid ganglioside.
Symptoms due to accumulation of ganglioside in nerve cells. Death usually occurs by 2-3 years old
Briefly outline skeletal muscle contraction
Electrical impulse crosses sarcoplasmic reticulum, resulting in Ca released. They bind to troponin causing change in tropomyosin, which exposes the actin-myosin binding site so its available for myosin to bind to.
ATP hydrolysed into ADP and Pi, allowing for the myosin head to cock and change shape, causing the filaments to slide over one another. Causes a power stroke as the actin filament releases energy, therefore myosin head recocks to original shape causing muscle contraction
What is exocytosis?
Material exported out of a cell.
(important in the secretion of numerous proteins including hormones, and extracellular structural proteins such as collagen and fluids such as mucus. )
How does exocytosis occur?
Involves fusion of vesicles from the interior of the cell with the plasma membrane. The vesicles contents are then expelled into the surrounding medium.
What are the different types of endocytosis?
- Phagocytosis
- Pinocytosis
- Receptor-medicated endocytosis
Briefly what is phagocytosis?(endocytosis)
Uptake of insoluble material in protozoa, also a form of feeding
also occurs in macrophages as defence
Briefly outline pinocytosis (endocytosis)
Cells pinch their plasma membrane to take up extracellular fluid in small vesicles.
Non-specific.