Week 7

Question 1

Gluconeogenesis

Answer 1

Net synthesis of “new glucose”
- reverse of glycolysis except: use 7 of the 10 enzymatic reactions of glycolysis, but in reverse direction
- therefore require other enzymes to bypass these reactions to produce glucose from pyruvate

Question 2

Irreversible glycolytic enzymes

Answer 2

• hexokinase
• phosphofructokinase-1
• pyruvate kinase

Question 3

Bypass enzymes of gluconeogenesis

Answer 3

1. Pyruvate carboxylase (ATP) - mitochondria
2. Phosphoenolpyruvate carboxykinase (GTP) - cytosol
3. Fructose 1,6-bisphosphatase - cytosol
4. Glucose-6-phosphatase - endoplasmes reticulum