Week 5 - Histo Methods - Downing

Question 1

Give me two words to describe each of these microscopy methods:
Light: 
Transmission: 
Scanning: 
Polarizing: 
Phase: 
Interference:
Fluorescence :
Confocal:

Answer 1

Light: basic, tissues
Transmission: cell, cytology
Scanning: 3d, surfaces
Polarizing: refractive indices along different optical axes
Phase: living, indices
Interference: living, like phase 
Fluorescence – UV, fluorescent 
Confocal : laser, 3d

Question 2

Basic tissue prep run-down:

Answer 2

• Fix the tissue
• Dehydrate with alcohol
• Clear by replacing alcohol with something tha can mix with wax
• Infiltrate and embed tissue with paraffin wax
• Section it in slices with microtome machine
• Stain if ya want

Question 3

Basic vs Acid staining? Give some charges here:

Answer 3

Basic - dye is attached to a (+) charge, will color stuff with a negative charge (like DNA)

Acidic - dye attached to a (-) charge, will color stuff with a (+) charge, (like proteins)

Question 4

Describe how hematoxylin/Eosin stains work:

Answer 4

Hematoxylin - a basic stain that will dye negatively charged stuff blue

Eosin will color all the positively charged stuff pink, because its an acidic stain

They are used together so you can see all the different cell componets

Question 5

Trimchrome stain used for?

Answer 5

Collagen

Question 6

What stains do you use for fats?

Answer 6

oil red, sun black

Question 7

Silver stains are sued to stain what?

Answer 7

stains the reticular fibers of connective tissue

will stain them black