Week 3

Question 1

Define and explain proteomics and interactomics in your own words, including techniques and
processes (eg. Trypsin-diges�on; RNA interference; TAP-tagging; site-directed mutagenesis; etc.)
* Describe the structure of nucleo�des and the manner in which these monomers are joined to
form a polynucleo�de strand

Question 2

Define transduction,and how each of these is used and contributes to
our understanding of the Central Dogma

Answer 2

Incorporating a gene into the genome by a viral vector and expressed. Foreign dna can be expressed too

Question 3

Define transfection,and how each of these is used and contributes to
our understanding of the Central Dogma

Answer 3

Naked DNA picked up, incorporated, and expressed. Foreign dna can be expressed too.

Question 4

Define transgenic, and how each of these is used and contributes to
our understanding of the Central Dogma

Answer 4

A foreign gene that’s been stably incorporated into a genome- foreign dna can be expressed too

Question 5

• Explain how site-directed mutagenesis, is used to contribute
  to our understanding of the Central Dogma

Answer 5

Modifying a gene to create a different protein- DNA alterations create different proteins

Question 6

Explain how knockouts, and is used to contribute
to our understanding of the Central Dogma

Answer 6

Mice lacking functional genes by genetic modification- No DNA for a gene=trait not being expressed

Question 7

Explain how RNA interference is used to contribute
to our understanding of the Central Dogma

Answer 7

DsRNAs break down mRNAs with identical sequences. Destroying mRNAs gets rid of proteins, no trait produced