Week 3

Question 1

Gram +/-

Answer 1

+ = single cell wall, purple; - = double cell wall, red

Question 2

Biofilm Formation

Answer 2

minutes = microbes attach to surface ; 2-4 hrs = microcolonies ; 6-12 hrs = protective matrix ; 2-4 days = colonies can rapidly recover w/i 25hrs

Question 3

Quiescence

Answer 3

hibernation

Question 4

Biofilm Formation: Stage 1

Answer 4

minutes, acquired pellicle,

Question 5

Biofilm Formation: Stage 2

Answer 5

coaggregation, microbes attract other microbes, able to withstand hydrodynamic forces

Question 6

Biofilm Formation: Stage 3

Answer 6

firmly attached, extracellular matrix, chronic disease established

Question 7

Biofilm Formation: Stage 4

Answer 7

mushroom colonies, specific species grow fast, fluid channels develop, quorum sensing (communication)

Question 8

Biofilm Formation: Stage 5

Answer 8

microbes disburse into other tooth surfaces