Week 2 RAS live Lecture 4

Question 1

1) What are the major group carriers with examples of their carriers?

Answer 1

1) Groups and their carriers
a) C1 = Tetrahydrofolate
C1 – methyl = S-methyladenosine
C1 – carboxyl (COO) = Biotin
C2 = Acetyl CoA
C3 = PEP
C5 = Isopentenyl pyrophosphate (IPP very reactive)
Amino = Glutamine/glutamate
Sulfur = Cysteine
Sugar = Nucleosidediphosphates
Complex (e.g. C-C-N) = glycine

Question 2

2) What are the difference units that can be carried on by Tetrahydrofolate (FH4)?

Answer 2

2) C1 can be carried by a variety of oxidation states:
a) Methanol
b) Formalaldehyde
c) Formic acid

Question 3

3) What is the source of CH3 and C1?

Answer 3

3) From serine and it is important for glycine pathways

Question 4

4) How is FH2 activated to FH4?

Answer 4

4) NADPH

Question 5

1) What does the activation of S-methyladenosine (CH3 donor) require?

Answer 5

5) ATP

Question 6

6) How is the CH3 activated?

Answer 6

6) Positive sulphur

Question 7

7) Where does CH3 come from usually?

Answer 7

7) From serine via FH4

Question 8

8) What is an example of C2 donation?

Answer 8

8) Fatty acid biosynthesis

Question 9

9) What is the carrier for phosphoenolpyruvate (PEP)?

Answer 9

9) No carrier – has built in energy source

Question 10

10) How is mevalonic acid constructed?

Answer 10

10) 3 acetyl CoAs

Question 11

11) What is the structure of S-adenosyl methionine?

Answer 11

11) adenine-ribose-methionine

Question 12

12) How is NH2 obtained?

Answer 12

12) add ATP to glutamine which forms glutamate and releases ADP and NH2