Week 2 OMK Objectives Flashcards

1
Q

List the three types of fuel found in the diet

A

Carbohydrates, proteins and fats

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2
Q

Identify healthy common sources of individual macronutrients (carbohydrates, proteins, fats, fiber)

A

Carbs: fruit, grains, legumes, veggies

Proteins: legumes, meat (chix, beef, pork)

Fats: nuts, fruit, dairy

Fiber: fruit, leafy greens, grains

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3
Q

Using the US Dietary Guidelines and MyPlate, select the general characteristics of a healthy diet, including the recommended contribution of various food groups and foods to be consumed in limited amounts

A

healthy diet includes fruit, vegetables, protein and grains and dairy. vegetables and grains should each take up over ¼ of the plate, and fruit and protein should each take up less than ¼ of your plate.

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4
Q

What are the recommended portion sizes for children, men and women for fruit, vegitibles, grains, protein, dairy, and oils?

A

Fruit: Children need ~2-3 cups, Women and Men need ~2 cups
Veggies: Children need ~1.5 cups, Women and Men need ~2-3 cups

Grains: Children need ~3-4 oz, Women and Men need ~3-4 cups

Protein: Children need ~5-6 oz, Women and Men need ~5-6 oz

Dairy: Children need ~3 cups, Women and Men need ~3 cups

Oils: Children need ~5 tsp, Women and Men need ~5-7 tsp

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5
Q

Describe MyPlate, explain how it could be used as a nutrition assessment tool, and give functional definitions of portion size in each category of the plate

A

MyPlate is an online resource that is aimed to promote American health and reduce overweight and obese persons by educating people about healthy food options. It emphasizes fruit, grains, vegetables, dairy foods, and proteins. It provides tools and resources to help people to develop healthy diets and track their individual diets and physical exercise.

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6
Q

Define Define Recommended Dietary Allowance (RDA)

A

The average daily dietary intake level that is sufficient to meet the nutrient requirements of nearly all (97-98%) the individuals in a life stage and gender group.

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7
Q

Define Adequate Intake (AI)

A

If sufficient scientific evidence is not available to calculate an EAR or RDA an AL is set. It is estimates of nutrient intake by a group of apparently healthy people that are assumed to be adequate.

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8
Q

Define Tolerable Upper Intake Level (UL)

A

The highest average daily nutrient intake level that is likely to pose no risk of adverse health effects to almost all individuals in the general population.

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9
Q

Define Estimated Average Requirement (EAR)

A

The average daily nutrient intake level estimated to meet the requirement of one half of the healthy individuals in a particular life stage and gender group.

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10
Q

what are the recommended Dietary Guidelines for Carbohydrates, fat, and protein

A
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11
Q

List the kcal/g for each of the macronutrients

A
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12
Q

Outline the relationship between food intake and energy output

A

The production of heat in the body increases as much as 30% above resting level during digestion and adsorption of food. Over 24 hrs, this may amount to 5-10% of total energy expenditure.

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13
Q

Differentiate between moderate and vigorous activity

A
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14
Q

Explain how varying levels of physical activity influence an individual’s nutritional requirements.

A

A sedentary person requires about 30-50% more calories than their RMR, however a highly active individual may require 100% more calories above their RMR.

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15
Q

Discuss the oxidation or synthesis of carbohydrates, proteins and fat in the red blood cell, adipose tissue, liver, brain and muscle during the fed state and after an overnight fast

A

RBC: The erythrocyte performs anaerobic glycolysis and pentose phosphate pathway for energy at all times (no mitochondria).

Adipose: In the fed state, insulin controls the absorption of glucose from various sources into adipose tissue. Triacylglycerides are synthesized and stored as fuel reserves. In the fasting state, triacylglycerols are degraded by hydrolysis, providing glycerol and fatty acids to the liver.

Liver: During the fed state, the liver stores glycogen, replenished proteins, and synthesizes triacylglycerols (fat), stored as Very Low-Density Lipoproteins (VLDL). In the fasting state, the liver degrades glycogen and performs gluconeogenesis. Oxidation of fatty acids supplies energy for gluconeogenesis, and acetylCoA for ketone body synthesis.

Brain: In the fed state, the brain uses glucose exclusively as a fuel source. In the fasting state, the brain can use either glucose or ketone bodies as fuel.

Muscle: In the fed state, muscle uses glucose as fuel, and increases protein synthesis to perform repairs. In the fasting state, the muscle can use fatty acids or ketone bodies from the liver as fuel. Protein in the muscle is degraded, and the resulting amino acids are used by the liver for gluconeogenesis.

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16
Q

Predict the ratio of glucagon/insulin in the fasting and fed states

A

Fasting: Glucagon ↑: Insulin ↓

Fed: Glucagon ↓: Insulin ↑

During the fed state, the high levels of insulin that are released act to promote storage of metabolic fuel, while the high levels of glucagon in the fasting state lead to promotion of breakdown of fuel stores which leads to, among other things, increase in blood sugar.

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17
Q

Describe the general structure and function of G protein-coupled membrane receptors and identify the general characteristics of second messenger systems associated these receptors

A

G protein coupled membrane receptor systems are tripartite:

1) A cell surface receptor
2) A G protein on the cytoplasmic face of the phosplipid membrane
3) An effector element (typically an enzyme or ion channel).

The surface receptor binds a ligand. This binding activates the G protein (often by replacing GDP with GTP), and the active G protein alters the activity of the effector element. This effector element changes the concentration of a second messenger within the cell. G protein coupled receptor systems often function to enact signal amplification, as the G protein can remain active for much longer than the time the ligand is bound to a receptor. The receptor itself (GPCR) is usually a seven-pass transmembrane protein (i.e. loops through the PM seven times).

Note: Glucagon activates a GPCR to perform its functions such as breakdown of liver glycogen, stimulation of gluconeogenesis, and stimulation of lipolysis (and then ketone body synthesis).

18
Q

Illustrate the general functions of receptor tyrosine kinases and receptor-associated tyrosine kinases

A

Receptor tyrosine kinases regulate various processes in the cell. A signal molecule binds to the receptor, which causes dimerization of receptor molecules. The dimer’s cytoplasmic domains have tyrosine kinases, which phosphorylate one another and become “active.” These active domains phosphorylate the tyrosine residues on various signaling proteins in the cell. This means a single RTK can modulate several different biochemical processes.

Note: Insulin is a signal molecule that activates a single class of RTK to cause “increased uptake of glucose and amino acids and to regulate metabolism of glycogen and triglycerides in the cell.”

19
Q

Describe the function, dietary sources, requirement, assessment, deficiency and toxicity for the water soluble vitamin thiamin.

A

Thiamin (aka B1)

Function: Required for carbohydrate metabolism; Prevents beri-beri disease

Sources: Whole and enriched grains, pork, wheat germ

Requirement: Adult Males: 1.2 mg/day; Females 1 mg/day

Assessment: Erythrocyte Transketolase Assay or High Performance Liquid Chromatography (HPLC) measurement of blood Thiamine Pyrophosphate (TPP)

Deficiency: Beri-beri (leg edema, heart failure, mental dementia, GI problems, muscle wasting) or Wernicke-Korsakoff (brain damage, disorientation)

Toxicity: Essentially nontoxic

20
Q

Describe the function, dietary sources, requirement, assessment, deficiency and toxicity for the water soluble vitamin niacin

A

Niacin

FUNCTION: Important in metabolizing proteins as coenzyme

SOURCE: Liver, meats, whole & enriched grains, nuts and legumes; tryptophan is a precursor

REQUIREMENT: M: 16 mg/day, F: 14 mg/day

ASSESSMENT: Urinary secretion of N1-methylnicotinamide

DEFICIENCY: Pellagrea (dementia, diarrhea, dermatitis, death)

TOXICITY: Large amounts may lead to flushing; “Higher doses of niacin (3000 mg/day) reduce LDL cholesterol 15 to 20% but may cause jaundice, abdominal discomfort, blurred vision, worsening of hyperglycemia, and precipitation of preexisting gout.” (Merck)

21
Q

Describe the function, dietary sources, requirement, assessment, deficiency and toxicity for the water soluble vitamin **riboflavin **

A

Riboflavin

FUNCTION: Helps conversion of carbs, fats, proteins into energy

SOURCE: Milk (& milk products), organ meats, eggs, leafy green veg, whole & enriched grains

REQUIREMENT: M: 1.3 mg/day; F: 1 mg/day

ASSESSMENT: Urinary excretion; erythrocyte riboflavin; Glutathione reductase activity

DEFICIENCY: Cheliosis (inflammation and fissuring at corners of the mouth, scaling of lip skin) or stomatitis (general inflammation of the mouth, incl gums, tongue, lips, palate); Magenta-colored tongue

TOXICITY: Essentially nontoxic

22
Q

Describe the function, dietary sources, requirement, assessment, deficiency and toxicity for the water soluble vitamin pantothenic acid

A

Pantothenic acid

FUNCTION: Part of coenzyme A, which is essential for metabolism of carbs and fatty acids, and synthesis of sterols

SOURCE: Organ meats, beef, egg yolks

REQUIREMENT: M&F: 5 mg/day

ASSESSMENT: Blood levels

DEFICIENCY: Rare

TOXICITY: Essentially nontoxic

23
Q

Describe the function, dietary sources, requirement, assessment, deficiency and toxicity for the water soluble vitamin biotin

A

Biotin

FUNCTION: Important in metabolism of carbohydrates & fats

SOURCE: Organ meats, eggs, milk, fish, nuts

REQUIREMENT: M&F: 25 mg/day

ASSESSMENT: Reduced urinary excretion of biotin; increased urinary excretion of 3-hydroxisovaleric acid

DEFICIENCY: Alopecia, bowel inflammation

TOXICITY: Essentially nontoxic

24
Q

Identify the factors that should be considered when determining a person’s caloric needs

A

Basal Metabolic rate: The energy needed by the body for basic functions

Dietary-induced Thermogenesis The energy the body needs to digest food, transport and absorb nutrients.

The thermic effect of activity: Most variable—based on physical activity of individual. Sedentary people thermic effect of activity could only total 15 percent of daily total energy expenditure; while athletes percentages may exceed 80%.

25
Q

Calculate BMI and use it to evaluate the body weight status of adults

A

BMI = kg/m2=(lbs/in2)x703

Weight Status

Below 18.5 Underweight

  1. 5 – 24.9 Normal
  2. 0 – 29.9 Overweight
  3. 0 and Above Obese
26
Q

Describe the major factors that contribute to the epidemic of overweight and obesity

A

Biological, social/psychological, cultural, and environmental influences

Excess calory intake (prevolence of sugary drinks, increased snacking, larger portion sizes, higher calorie-density of food, eating out, advertizing)

Inadequate physical activity (labor saving technology, media use, motorized transportation, limited recreational space, limited time for child PE and recess)

Instant gratification

27
Q

Define microbiome or normal flora

A

Normal Flora: Bacteria found in or on our bodies on a semi-permanent basis which don’t cause disease (unless additional pathology occurs).

28
Q

Identify a potential role of the human microbiome in maintaining human wellness

A

Native bacteria impact not only which dietary components their host is able to extract from its diet, but also how those dietary components are converted into biological signals. Gut microbes also impact host energetics.

Gut microorganisms benefit the host by extracting the energy from the fermentation of undigested carbohydrates and the subsequent absorption of short-chain fatty acids. The most important of these fatty acids are butyrates, metabolised by the colonic epithelium; propionates by the liver; and acetates by the muscle tissue. Intestinal bacteria also play a role in synthesizing vitamin B and vitamin K as well as metabolising bile acids, sterols and xenobiotics.

Imbalanced bacterial communities in the gut have been associated with metabolic disorders like obesity; gastrointestinal disorders like Crohn’s disease and colitis; the inflammatory reactions underlying asthma.

Cummings, J.H.; MacFarlane, G.T. (1997). “Role of intestinal bacteria in nutrient metabolism”. Clinical Nutrition 16: 3–9. doi:10.1016/S0261-5614(97)80252-X. PMID 16844615.

29
Q

Explain the term “generation time” and the various factors that can affect it.

A

the interval of time that elapses between one generation of bacteria and the next.

The term may be thought of as a quantitative parameter measuring reproductive output rate, where, bacterial species with access to unlimited resources will divide at a constant rate.

30
Q

Describe the concept of quorum sensing and its importance.

A

In close proximity, bacterial cell secretion of chemical signals permits cell to cell communication. As the cellular aggregation grows, the concentration of a specific signaling molecule, referred to as an autoinducer (in the microbio textbook), becomes great enough such that the cells in the biofilm coordinate activities such as adhering more tightly to one another or to a surface.

Coordinated activity of the biofilm underpins the importance of quorum sensing, which has been implicated in increased antibiotic resistance. When in a biofilm, cells may coordinate secretion of protective sugars with which to surround themselves and through which antibiotic drugs have difficulty penetrating. Quorum sensing may also cross the boundary between eukaryotic and prokaryotic cells.

31
Q

Describe the morphology and arrangement of bacterial cells using acceptable scientific terms (cocci, bacilli, etc).

A

Bacilli (pl) Bacillus (s.) - Rod shaped

Cocci (pl.) Coccus (s.) - Sphere shaped

Spirochete - Spiral shaped

32
Q

List some important gram-positive bacteria and their morphology and arrangement.

A

Gram positive bacteria have thick peptidoglycan layers in their cells walls and appear purple in the staining procedure. (Retention of crystal violet dye)

Streptococcus pyogenes - Causes “strep throat”. Is a Gram (+) coccus.

Propionibacterium acnes – Gram (+) bacillus (rod). It causes pimples

Staphylococcus epidermidis - Gram + coccus. Lives in the skin and can cause infection in immune compromised patients.

Streptococcus pneumoniae - Gram (+) cocci . Caises pneumonia.

33
Q

List some important gram-negative bacteria and their morphology and arrangement.

A

Gram negative bacteria have little peptidoglycan in their cells walls and appear pinkish or reddish in the staining procedure (crystal violet is washed out). Gram negative bacteria typically have an inner phospholipid bilayer and an outer phospholipid bilayer

Escherichia coli (E. coli) - Gram (-) rod. Can cause various infections.

Salmonella

34
Q

List some important acidfast bacteria and their morphology and arrangement.

A

Acid Fast bacteria are neither gram+ nor gram-. They are named acid fast for the staining procedure used to identify them. In general, their cell wall properties differ from gram stained bacteria. Their cell walls contain mycolic acid.

Mycobacterium tuberculosis - Acid fast bacterium that causes TB.

Mycobacterium leprae - the cause of leprosy

35
Q

Describe where teichoic acids are found and their importance.

A

Links the multiple layers of peptidoglycan found in Gram-Positive bacteria cell walls

Importance/ function: Reinforces the multiple peptidoglycan layers of the Gram-Positive Cell Envelope.

36
Q

Describe the components and functions of the outer membrane of Gram-negative bacteria.

A

Overall Function - defense and has toxigenic properties on many pathogens

Inward-Facing Leaflet: has a phospholipid composition similar to that of inner membrane.

Includes lipoproteins, function is to connect outer membrane to peptide bridges of cell wall.

       Major lipoprotein: Murein Lipoprotein (or “Braun lipoprotein - see image).

Without murein lipoprotein, as cell grows and divides, outer membrane will not attach to the growing cell wall, causing outer membrane to balloon out into where daughter cells separate

Outward-Facing Leaflet: main phospholipids are called lipopolysaccharide (LPS)

LPS have shorter fatty acid chains than those of inner membrane, some are branched

Function - acts as an endotoxin, which is a cell component that is harmless as long as pathogen remains intact. When endotoxin is released by a lysed cell, it overstimulates host defenses. Antibiotic treatment of LPS-containing pathogen can kill cells, but can lead to death of patient

Other Proteins of Outer Membrane: unique to outer membrane, not found on inner.

Porins - permit entry of nutrients (sugars and peptides). They are a cylinder of beta sheets. Typically exists as a trimer of beta barrels, each act as a pore for nutrients.

Lack specificity, so can updatake various molecules including antibiotics.

37
Q

Describe and give examples of live vaccines

A

Contain live, but attenuated (weakened) viruses. Made most commonly by passing the disease-causing virus through a series of cell cultures or animal embryos.

Risk: mutations can occur when the vaccine virus replicates in the body to become more virulent. Ex: mutations common in oral polio vaccine - but this is no longer used.

Viral DiseaseSource: Chickenpox, Measles/ Mumps/ Rubella (MMR), Yellow Fever, Zoster

Bacterial DiseaseSource: Tuberculosis

38
Q

Describe and give some examples of inactivated vaccines

A

Inactivated VaccineSource:

Also known as killed vaccines. Created by inactivating a pathogen, typically by using heat or chemicals, destroying the pathogen’s ability to replicate, but keeps it “intact” so the immune system can recognize and form antibodies to it.

Viral DiseaseSource: Hep A, Influenza, Polio, Rabies

Bacterial DiseaseSource: Typhoid Fever, Typhus

39
Q

Describe and give some examples of toxoid vaccines

A

Toxoid VaccineSource:

Since some bacterial diseases are not directly caused by a bacterium, but by a toxin produced by the bacterium, immunization can be made by inactivating the toxin that causes the disease symptoms. These vaccines are created similarly to those of inactivated vaccines, and are called toxoids.

Viral DiseaseSource: None (caused by toxins from bacteria)

Bacterial DiseaseSource: Anthrax, Cholera, Diphtheria, Tetanus

40
Q

Describe and give some examples of subunit vaccines

A

Subunit VaccineSource:

Contain only pieces of the pathogens they protect against. Created by isolating a specific protein from pathogen and presenting it as an antigen.

Viral DiseaseSource: Hep B, Influenza

Bacterial: Pertussis, Hib (meningitis), Lyme Disease, Meningococcal, Pneumococcal pneumonia, Human Papillomavirus (HPV)

41
Q

Identify health promoting behaviors

A

Lifestyle: your physical activity and healthy behaviors
Nutrition: eat right! Up Omega 3 Fatty Acids, avoid bad carbs, etc.

Family History: Know what diseases run in your family

Mind-Body: stress management, emotional health, and social connection

Spirituality: Finding a connection that results in meaning and purpose in your work.