Week 2 Lab: Screening Test for Phagocytic Engulfment Flashcards

1
Q

True or False: No special preparation of the patient is required before specimen collection.

A

True

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2
Q

Blood should be drawn by an _____ technique.

A

aseptic

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3
Q

Blood sample: A minimum of ______________ or ______________ are required.

A
  • 2 mL of heparin blood (green-top evacuated tube)
  • 15 to 20 heparinized capillary tubes
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4
Q

After blood collection, the specimen should be _________.

A

centrifuged

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5
Q

For the quality control, _________________ should be tested simultaneously.

A

a fresh, heparinized sample of blood from a healthy volunteer

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6
Q

Materials for the experiment?

A

• Broth culture of Bacillus subtilis or Staphylococcus epidermidis
• Microscope slides
• Pasteur pipettes
• Rubber bulb
• Test tubes
• Wright’s stain

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7
Q

What are the labels of the 2 test tubes?

A

Patient test tube and Control test tube

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8
Q

Add ______ drops of the buffer coat from either blood sample to the respectively labeled tubes.

A

4 to 8 drops

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9
Q

Add ______ drops of the bacterial broth culture to each tube.

A

2 to 3 drops

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10
Q

Incubate both tubes at room temperature or _____ for _____.

A

37C; 30 minutes

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11
Q

Place _____of the incubated specimen on a glass slide and prepare a smear.

A

1 drop

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12
Q

Air dry the slides and stain with _________.

A

Wright’s stain

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13
Q

Cover each smear generously with filtered Wright’s stain and allow the stain to remain on the slide for at least _________.

A

5 minutes

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14
Q

Slowly add distilled water or buffer to the stain until the buffer begins to overflow the stain. Watch for the appearance of a _________.

A

metallic luster

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15
Q

Gently _____ on the slide to mix the stain and buffer.

A

blow

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16
Q

Allow the buffer to remain on the slide for at least _________.

A

5 minutes

17
Q

Gently wash the stain and buffer off the slide with ___________.

A

distilled water

18
Q

Air dry or carefully blot the slide between two sheets of ___________.

A

bibulous paper

19
Q

Interpretation of Results: Positive?

A

Demonstration of the engulfment of bacteria

20
Q

Interpretation of Results: Negative?

A

No engulfment of bacteria

21
Q

It is the process by which specialized cells engulf and destroy foreign particles such as microorganisms or damaged cells.

A

Phagocytosis

22
Q

___________ are the most important phagocytic cells.

A

Macrophages and neutrophils (PMNs)

23
Q

A mixture of bacteria and phagocytes is incubated and examined for the presence of engulfed bacteria. This simple procedure may be useful in supporting the diagnosis of ___________________ in conjunction with clinical signs and symptoms.

A

impaired neutrophilic function

24
Q

This procedure may produce ____________ if the blood specimen is not fresh or if a coagulase-positive Staphylococcus specimen is used.

A

false-negative results

25
Q

This procedure may produce false-negative results if _______________.

A
  • the blood specimen is not fresh
  • if a coagulase-positive Staphylococcus specimen is used
26
Q

In Addition, the bacteria must be ___________ for the test to be positive.

A

intracellular and not extracellular

27
Q

The failure of phagocytes to engulf bacteria can support the diagnosis of __________; however, these results must be used in conjunction with patient signs and symptoms.

A

neutrophilic dysfunction

28
Q

True or False: This is the simple screening procedure for engulfment. The presence of engulf bacteria does not demonstrate that the bacteria have been destroyed.

A

True