Week 2: Introduction to HIV/AIDs Flashcards
What is the supposed origin of HIV1/2?
Sporadically spread from non-human primates to primates.
Particularly, gorillas and chimpanzees for HIV1 and the sooty mangabey for HIV2
What are the different lineages of HIV1?
Four distinct lineages M-P, each lineage will have following variants and mutants
M has the most distinct variants
Each lineage is more common in certain geographic regions
What are the different lineages of HIV2?
Eight different lineageas A-H, each with following variants and mutants
Each lineage is more common in certain geographical regions
How are HIV1 and 2 related?
Share a common ancestor - similarities
Also have differences in evolutionary origin resulting in distinct features
What are the main differences between HIV1 and HIV2?
HIV1 is more common over 90% of cases, whilst HIV2 is rare and mainly only present in West Africa and India
HIV-2 has a lower transmissibility, less likely to cause AIDs mainly due to lower plasma viral loads so less infection of CD4+ cells
Immune response against HIV2 is stronger - higher levels of IL-2 and humoral responses including neutralisation.
What are the main similarities between HIV1 and 2?
Same modes of transmission
Same intracellular replication process
Same clinical features
Where is the majority of the HIV burden felt?
Sub-saharan Africa (considered endemic)
White homosexual males
Black heterosexual females and children
Give an overview of the biology of HIV/AIDs?
HIV infects mainly CD4+ T cells, resulting in progressive loss of T helper cells and immunodeficiency develops.
Patient develops characteristic cancers and infections
Leads to death over approximately ten years if left untreated.
In some individuals disease may be eliminated but treatment mainly slows the progression of disease
Describe how treatment of HIV has changed over time?
1994 - zidovudine prevent mother child transmission
1996 - ART blocks replication of HIV
2004 - global plans to roll out ART and reduce AIDS develops
2009 - HIV vaccine provides partial protection to selected individuals and stem cell transplants cure an infected HIV patient
(low importance)
Describe how the diagnosis of HIV has changed over time?
1959 - first document HIV case (not known at time)
1981 - immunodeficiency in homosexual men noted
1983 - HIC linked to AIDs
1984 - HIV antibody assay developed
1995 - HIV latency described
What is the prevalence of HIV like in UK?
white gay/bi men
Black african heterosexuals (more women)
1 in 3 living with HIV are aged above 50yrs (longer life expectancy and delay before diagnosis)
Majority are diagnosed between 35 and 49
Describe the structure of a HIV virus
Lipid envelope (with gp120) and matrix
Capsid - containing p24
Has two identical copies of SS viral RNA - often dimerised together to increase stability and more effective for RNA replication
Contains reverse transcriptase, integrase and protease enzyme
What receptors are needed for HIV invasion and replication in a host cell?
Primarily - CD4+
Co-receptors - CCR5 and CXCR4
Binds to HIV attachment proteins gp120
How do the receptors HIV requires for infection link to the tropism of the virus?
CD4+ on T helper cells (effector and memory), macrophages and dendritic cells
CCR5 (most common co-receptor in initial infection 0 - T cells, GALT, Macrophages and DC
CXCR4 - mainly only on T cells (late stage infection)
How does the stage of HIV infection affect the co-receptor used?
Initial infections - typically CCR5
Late infections - typically CXCR4
Describe the replication process of HIV within the cell?
gp120 on lipid envelope binds to primary receptor CD4 on T helper cells (MC and DCs)
second gp120 binds to co-receptor CCR5 (CXCR4)
Causes fusion with cell membrane, releases content into host cytoplasm, capsid fuses releasing viral RNA and proteins
Protease - hydrolysis protease bond in pre-cursor proteins to create functional proteins
Reverse transcriptase - creates pro-viral DNA from RNA
pro-viral DNA enters the nuclease and is inserted into the host genome by integrase
Host machinery produces new viral RNA and used to make viral proteins
Viral proteins move to cell surface and assemble to form new viral particles, which are released surrounded by part of the host cell membrane.
Cleaves glycoprotein forming a mature virus
How does the immune response against HIV actually aid the progression of the virus?
1.HIV pro-viral DNA inserted into host genome. Cytokine receptors activated on host T cell - triggers replication of genome including HIV gene leading to higher levels of HIV in the cell - cell death
- HIV infects DC, migrates to lymph node - abundant supply of host cells to infect
- Proliferation of activated T cells in the periphery increases the target number of Cells for HIV.
What are the different phases of HIV infection?
When?
Eclipse phase: 0-3 weeks
Acute phase: 3 to 9 weeks
Chronic phase: 4 to 6 months onwards