Week 1: Immunology workshop Flashcards
What are the histological features of fibroblasts?
Active - spindle shaped cell, often only nucleus is visible darker stained and ovoid in appearance
Inactive (fibrocyte) - elongated shaped cell, nucleus is smaller, flattened, elongated and more darkyl stained
Cytoplasm is highly eosinophilic typically indistinguishable from surrounding collagen.
What is the function of a fibroblast?
Permanent residents of connective tissue, that produce and secrete the ECM, mainly collagen and elastin.
Some differentiate into myofibroblasts which have a role in wound contraction hence and wound healing.
What tissue resident cells should be identifiable in connective tissue?
Fibroblasts
Macrophages
What does collagen look like under histological imaging?
Typically pink bundles
Often associated with fibroblast nuclei
What are the histological features of a monocyte?
Largest of white blood cells
Large eccentrically placed kidney bean shaped nucleus.
Abundant cytoplasm with some fine granules
Draw diagrams to represent the histological appearance of neutrophils, lymphocytes, monocytes/macrophages, eosinophils, basophils and plasma cells.
Plasma cells - large eccentric round nuclei, with clock face chromatin
What are the histological features of cardiac muscle?
Straited
Intercalated disks
Single centrally located round nucleus
Branched appearance appearance
What are the histological features of skeletal muscle?
Eosionophilic cytoplasm
Many peripheral nuclei
Striated
Unbranched structure
Fibrocollagenous septa containing blood vessels
What are the histological features of smooth muscle?
Single Centrally located nucleus
Not striated
Spindle shaped cells
Gap junctions
How does the regenerative capacity of the different types of muscle vary?
Cardiac muscle - permanent, no regeneration
Smooth muscle - stable regenerates by mitosis
Skeletal muscle - stable, satellite cells (small population of stem cells)
Describe the histology of neural tissue?
Nerve axons (eosinophilic), typically surrounded by a myelin sheath (thicker indicates more insulation), may identify nuclei on the edge of this sheath belonging to the shwann cells.
What is the basic histological structure of the gall bladder?
Consists of:
Mucosa: Epithelium - simple columnar with prominent folds till the bottom of the lamina propria.
Lamina propria - may contain crypts of epithelium
Muscularis mucosa - quite thick
Submucosa:
Muscularis externa:
Serosa:
What are the science names for gall stones?
Cholithiasis in the gall bladder
Choledocholithiasis - in the bile duct
Describe the histological features of the lung parenchyma/alevoli?
Thin walled - type 1 and type 2 pneumocytes
Type 1: thin walled simple squamous epithelium with a flattened nucleus
Type 2: septal cells, source of surfactant, round and bulge into alveolar spaces
Walls around a large air spaces, surrounded by smaller capillaries marked by smaller lumens and singular flattened nuclei.
What are the key microscopic features of acute inflammation?
Neutrophil migration
Oedema - tissue exudate
Macrophage proliferation
Vascular congestion - indicate slowing of blood flow and coagulation.
Made see indication of vasodilation in widened spaces between endothelial cells.
What are the possible outcomes of acute inflammation?
- Progression to chronic inflammation is damaging stimuli is not removed
- Damaging stimuli removed and regeneration (return to normal function)
- Damaging stimuli removed and healing by fibrosis (scar) (no functional)
- A combination of repair and regeneration can lead to partially functional tissue
What are the key features of chronic inflammation?
Inflammatory damage and attempts at repair occur simultaneously
Identify macrophages and lymphocytes in infiltrate
Collagen, fibroblasts indicate fibrous tissue (scar) formation, this changes the ECM where there was previously parenchymal or stromal tissue.
Granulation tissue
What is the key outcome of chronic inflammation?
Typically: healing by fibrosis resulting in loss of tissue function
What are the key histological features of granulomatous inflammation?
Macrophages - epithelioid cells, foam cells, Multinucleate giant cells and activated macrophages
These ring around a core containing the pathogen, is often also necrotic
May be surrounded by an outer ring of lymphocytes
May be an additional outer ring of fibrosis
What are the factors that affect wound healing?
Extreme age
Immunocompromised - HIV
Diseases - diabetes
Low perfusion - diabetes or obesity
Persistent damaging stimuli - infection
Mechanical forces - pulling open wound or picking scab
Nutritional status
Poor wound hygiene
Fibrin coating (supports healing)
necrosis (can harbour pathogens).