Week 2 Histopathology Flashcards
What type of cells are the arrows pointing at? What is this a slide of?
Kupffer cells are simply macrophages of the liver, but the principle of phagocytosis of a vital dye is similar to histological staining
What is this a slide of and what is the arrow pointing to?
In the lung, macrophages or “dust cells” are responsible for scavenging the interior of the alveolar septum and the surface of the alveolus.
What role can macrophages play in congestive heart failure?
In congestive heart failure, the lungs become congested with blood (increasing capillary pressures), erythrocytes then pass into the alveoli from ruptured capillaries, where they are phagocytosed by alveolar macrophages. These hemosiderin-laden cells are sometimes called “heart failure cells”.
What are these cells, how do you know and what is their role? What do these primarily secret?
hese slides of mammary glands were stained with toluidine blue to reveal the granules (the granules change the color of the stain) of mast cells. They have a specific role in allergic responses and they secrete histamine
What are these cells and why would there be a high concentration of them in the duodenum?
Plasma cells (Green Circle), are identified by their characteristic meta-chromatic ovoid shape, eccentrically placed nucleus, and peri-nuclear clear areas. The gut is an area where high amounts of bacteria attempt to enter and these cells have a large role in immune response.
What cell is the arrow pointing at? What are these cells easily confused with?
Fibroblasts (Red Arrow), with elongated nuclei and cell bodies, can also be found in the mast cell and duodenum slides. Note the fibroblasts forming a “sheath” along the inside of the villus beneath the basement membrane, separating the epithelial cells and connective tissue of the villus. These cells can easily be confused with the squamous epithelial cells found right next to them.
What is this a slide of and what are the elastic fibers synthesized from? What is the blue coloring and what is in the reddish brown color?
What cells synthesize elastic fibers?
What type of collagen is found in this tissue?
With the Quad stain, elastic fibers will appear reddish brown. In the H & E stain, the elastic sheets are very thick and the elastin has a relaxed wave-like conformation due to the collapse of the vessel wall (Ross and Pawlina, plate 4). Elastic fibers in the aorta are synthesized by smooth muscle cells. Collagen type I is blue and the red brown color is elastic fiber.
Fibroblasts and vascular smooth muscle cells create elastic fibers
Type I collage is prominent in blood vessels, smooth muscle and loose connective tissue
What is this a slide of and what are the arrows pointing at?
Reticular fibers can only be observed when this silver stain is used, and are prominent in highly cellular organs, such as the lymphoid organs: spleen, liver, lymph nodes, and adipose tissue. In the following arrangements of connective tissue, notice the relative proportions of cells, fibers, and other extracellular material.
What is this a slide of and what type of tissue is present? How do you know?
In this example of mucous connective tissue (also known as Wharton’s jelly), note the preponderance of ground substance relative to the number of cells. The immature fibroblasts (mesenchymal cells) are less elongated than in other types of connective tissue and appear “amoeboid” in places. This is a slide of an umbilical cord.
What is this a slide of and what is highly found in this tissue?
This is thick skin and it contains high amounts of collagen
What is this a slide of? What is this primarily made up of?
In tendon, the fibers and fibroblasts are arranged in a regular pattern; note the high fiber/cell ratio. Tendon has primarily collagen fibers.
What type of cells are the black arrows pointing at?
Macrophages - these cells stain lighter and have compontents in their cytoplasm
