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Gene Tech > Week 11 > Flashcards

Week 11 Flashcards

1
Q

Fab domain of antibody

A

Binds to epitope

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2
Q

Fc domain of antibody

A

Biological effector

Modulate protein function

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3
Q

How are monoclonal antibodies made using mouse?

A

Antigen injected into mouse —> immine response harvested —> mix with immortalised myeloma cells —> fused into hybridoma cells (via salvage pathway) —> HAT medium selection

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4
Q

Monoclonal antibody production (no mous)?

A

Cell in well with growth factor —> antibodies secreted in medium —> test reaction w antigen —> propagate positive —> isolate and purify

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5
Q

Humanising process (3)

A

1 100% mouse antibody

2 chimera. Human comstant regions, mouse variable regions.HAMA response reduced

3 mostly human, HAHA response. CDRs only from mouse.

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6
Q

Phage display

A

Isolate human b cells and transfect into animals

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7
Q

Human system for antibodies

A

Find survivor of disease and try to immortalise b cell to find antibody

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Gene Tech (12 decks)

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