WEEK 1 Flashcards
Surgical specimen should be …
fixed asap
Histopathology refers to …
microscopic examination of tissue
Histopathology in clinical medicine refers to
the examination of biopsy
All tissues must be
labeled and identified
Tissues should be
taken at right angle
Autopsy material should be
fixed as soon as possible or placed in a mortuary refrigerator (4 degree C)
The thickness of tissue specimen
5mm
The thickness for lung tissue specimen
1-2 cm
What specimen are must be kept for possible cultures
Purulent materials, exudates, transudates
The amount of fixatives
20-50 times the volume of tissue specimen
The amount of fixatives for prolonged fixation
50-100 times the volume of tissue specimen
Action for hollow organs
Should be packed with cotton soaked in fixation
Only use of one eyepiece
Monocular heads
Only use of two eyepiece
Binocular heads
Only use of three eyepiece
Trinocular heads
Also known as compound light microscope
Bright field microscopy
To observe unstained and transparent specimen
Dark field microscopy
Dark field microscopy
To observe unstained and transparent specimen
An optical microscopy illumination technique
in which small phase shifts in the light passing through a transparent specimen are converted into amplitude or contrast changes in the image.
Phase contrast microscopy
A contrast-enhancing technique that improves the quality of the image obtained with birefringent materials when compared to other techniques such as bright field microscopy, phase contrast microscopy and dark field microscopy.
Polarized light microscopy
It is best for TB disease identification
Fluorescence Microscope
A microscope that uses a beam of accelerated
electrons as a source of illumination.
Electron microscope
Electron microscope
A microscope that uses a beam of accelerated
electrons as a source of illumination.
It allows 2D visualization
Transmission electron microscope
It allows 3D visualization
Scanning Electron Microscope
