WBC COUNTING Flashcards
Parts of wbc pipette
Calibrated stem (1 unit)
Bulb (10 units)
Bead (white)
Stem
Materials and equipment
Anticoagulated blood
Wbc pipette
Diluting fluid
Tally counter
Counting chamber
Gauze pad
Microscope
Test tube
Aspirate blood up to what mark of the capillary stem?
0.5 mark
Dip the pipette in to the diluting fluid and draw diluting fluid until it reaches the
11 mark
Using ___ count the wbcc in the 4 large squares
Lpo
Each wbc squares is divided further to
16 squares
Leukocyte count/cu.mm =
of wbc counted in 4sq.mm x area cfx depth cf ×dil. cf
Area correction factor
1/4 or 4
Depth of countjng chamber
0.1 ot 1/10 mm
Depth correction factor
10
Ratio of dilution
1:20 or 1/20
Dilution correction factor
20
1 cu mm = ?
1 ul
If px has leukopenia draw blood to ___ and diluent to ___
1 mark
Diluent: 11