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Hematology 1 > RBC COUNTING (LAB) > Flashcards

RBC COUNTING (LAB) Flashcards

1
Q

The number of red cell in 1 cu. mm

A

Red cell count

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2
Q

The red cell count is the number of red cells in ____.

A

1 cubic millimeter

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3
Q

The rbc count is one of the test that are used for the diagnosis of ___ and ____.

A

Anemia
Polycythemia

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4
Q

Parts of RBC pipette

A

Calibrated stem (1 unit)
Bulb
Bead (red)
Short stem

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5
Q

Materials and equipment used in RBC counting

A
  • anticoagulated blood
  • RBC pipette
  • Diluting fluid
  • Tally counter
  • Counting chamber
  • Gauze pad
  • Microscope
  • Test tube
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6
Q

The RBC pipette should be filled with blood up to?

A

0.5 mark

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7
Q

Used to wipe the outside walls of the pipette

A

Gauze pad to avoid contamination

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8
Q

Gently rotate the pipette to mix the ____ and ____.

A

Diluting fluid and blood

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9
Q

Mix for how many minutes?

A

5 minutes

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10
Q

How many drops of the diluted sample must be discarded

A

3-4 drops

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11
Q

Position of the hemocytometer in the microscope stage

A

Horizontal position

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12
Q

1 large square is made up of how many squares?

A

9 (1mm x 1mm) squares

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13
Q

Each WBC squares is divided further into how many squares?

A

16 squares

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14
Q

The center square for RBC is subdivded into

A

25 smaller squares

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15
Q

Distance between each counting chamber and coverslip (depth)

A

0.10 millimeter

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16
Q

Total volume is?

A

9 cubic millimeters

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17
Q

Calculate the number of RBC per___

A

Per liter of each side of the hemocytometer

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18
Q

Total area counted for RBC counting

A

1/5 square millimeter (mm²)

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19
Q

How many parts of blood and how many parts of diluting fluid is needed for the dilution?

A

1 part of blood to 200 parts diluting fluid
1:200

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20
Q

To calculate the rbc count per cubic milliliter of blood, simply multiply the _____

A

Number of rbc counted x area correction factor x depth correction factor x dilution correction factor

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21
Q

The answer is expressed in

A

Millions cells per cubic milliliters of blood sample

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22
Q

We can convert it in SI unit which is ___

A

Number of cells x 0.000001 =
_____ x 10¹²/L of blood

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23
Q

Depth counting chamber

A

0.1 mm

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24
Q

Depth of counting chamber in fraction

A

1/10 mm

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25
Q

Depth correction factor

A

10

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26
Q

How many parts of blood

A

1 part of blood

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27
Q

How many parts of diluting fluid

A

200 parts

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28
Q

Dilution factor for rbc ct

A

200

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29
Q

Area correction factor for RBC counting

A

5

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30
Q

Length of one small square

A

1/5 mm or 0.2 mm

31
Q

Width of one small square

A

1/5 mm or 0.2 mm

32
Q

Area of one small square

A

1/5 mm x 1/5 mm = 1/25 mm² or 0.4 mm²

33
Q

In Rbc counting, we count in how many small squares?

A

5 small squares

34
Q

Total area of 5 small squares

A

1/25mm² X 5 = 1/5 mm²

35
Q

Variation in technique

A
  • polycythemia or erythremia
  • anemia
36
Q

If px has polycythemia, blood is drawn to what mark? the diluent is up to what mark?

A

0.3 mark of the rbc pipette and diluent up to 101 mark

37
Q

Final dilution if the px has polycythemia

38
Q

If the px has anemia, up to what mark of the rbc pipette and diluent is aspirated?

A

1 mark and the diluent to 101

39
Q

Final dilution if the px has anemia

40
Q

Reference values

A

Normal values:
Female: 4.0 - 5.4x 10¹²/L
Male: 4.6 - 6.0 x 10¹²/L

41
Q

Physiologic Variation

A
  1. Increased count in dehydration
  2. Increased count in exercise
  3. Newborn children have higher counts than adults
  4. Women have lower counts than male
  5. Individual living at higher altitudes have higher counts
42
Q

Dehydration results in

A

Decreased plasma in blood

43
Q

Pathologic Variation

A
  1. Increased erythrocyte count in polycythemia
  2. Increased in pulmonary tuberculosis and pulmonary fibrosis
  3. Increase in acute poisoning
  4. Decrease in anemia and after hemorrhages
44
Q

Excessive amount of rbc makes the blood

A

Thicker than normal

45
Q

Rules in counting

A
  1. Observe the boundary line of the square
  2. Never count cells drying preparation
  3. Never count cells underfilled or overfilled chamber
46
Q

2 boundary lines of the square

A

A. Double line - outer line is the boundary of the square
B. Triple Line - middle line is the boundary of the square

47
Q

Counting rule
• Do not count cells touching the

This is to avoid double counting

A

Bottom line
Right line

48
Q

Composition of hayem’s fluid

A

0.5 g of mercuric chloride (antiseptic)
1 g of Sodium chloride (provides isotonicity)
5g of sodium sulphate (prevents rouleaux formation)
200 ml of distilled water (solvent)

49
Q

Locate the small square using what objective lens?

A

Low power objective

50
Q

To visualize the fine lines, adjust the ____, and switch to 10x then 40x

A

Light intensity

51
Q

In what specific smaller squares we will count?

A

1st, 5th, 25th, 21st, 13th

52
Q

Rule that we use in counting

53
Q

5 smaller squares is equal to how many smallest squares?

A

80 smallest squares

54
Q

1 smaller square is equal to how many smallest squares?

A

16 smallest squares

55
Q

Conditions under Low rbc count/ erythropenia

A

Anemia
Vitamin B deficiency
Internal bleeding
Kidney diseases
Malnutrition

56
Q

Conditons under High rbc count/ polycythemia

A

Smoking
Congenital heart disease
Dehydration
Hypoxia
Lung disease

57
Q

Platelet count diluting solution

A

1% ammonium oxalate
1g ammonium oxalate in 100ml of distilled water

58
Q

If platelet count is extremely low, use the ___ pipette then follow the formula for ___ count using 1% ammonium oxalate as diluting fluid.

59
Q

Normal platelet count ranges from

A

140,000 - 450,000 cells/cu.mm

60
Q

Conditions associated in Low platelet count/ thrombocytopenia

A

Leukemia and certain other cancers
Certain Types of anemia
Certain Viral infection
Certain Respiratory disorders
Radio and chemotherapy
Sepsis
Certain autoimmune disorders
Certain medications and drugs
Exposure to toxic substances
Alcoholism
Cirrhosis
Iron and folate deficiency
Vitamin B12 deficiency

61
Q

Conditions associated with High platelet count/thrombocytosis

A

Bleeding
Cancer
Certain infections
Iron deficiency
Removal of spleen
Certain types of anemia
Certain inflammatory diseases
Surgery

62
Q

Rbc pipette has ___ bulb than Wbc pipette

63
Q

Wbc pipette has ___ lumen than rbc pipette

64
Q

thick slide with h-shaped groove

A

Neubauer slide

65
Q

composition of cover slip and neubauer slide

A

Neubauer chamber

66
Q

collection of materials that we need to use

A

Hemocytometer

67
Q

used in counting rbc and wbc

A

Thoma pipette

68
Q

Diluting fluid for rbc

A

Hayem’s fluid
0.85% normal saline solution
Toisson’s Fluid

69
Q

Purpose of RBC Diluting fluid

A

use to preserve the integrity of the rbc

70
Q

In wbc, diluting fluid is used to ___

A

lyse the rbc for better reading of wbcs

71
Q

Function of Tally counter

A

Used for rbc counting and sperm analysis

72
Q

Discarding 3 to 4 drops of the diluted sample just to make sure that the sample charged in the chamber is a ___.

A

mixed solution (rbc and diluting fluid)

73
Q

one of the criteria for a quality rbc counting is

A

Making sure that the chamber is not overfilled or underfilled

74
Q

We have half way in and half way out and it is only applicable in the

A

upper line and left line

Hematology 1 (5 decks)

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