W6C12T4.3 Transcription Flashcards
What is transcription?
Making complementary RNA to template DNA.
Which enzyme carries out transcription?
RNA polymerase
RNA polymerase function
Adds successive nucleotides to 3’ end of growing transcript
First stage of transcription
Initiation
What happens during initiation
DNA strands separated, transcription of template strand begins
Second stage of transcription
Elongation
What happens during elongation
RNA polymerase adds nucleotides to 3’ end of growing RNA transcript
Third stage of transcription
Termination
Transcription starts at a (1) and ends at a (2)
- promoter
- terminator
Promoter also called
TATA box: sequence 5’-TATAAA-3’
Mediator complex
Complex of proteins that recruit RNA polymerase to the promoter; before initiation of transcription
Primary transcript
RNA transcript that comes off template DNA strand
mRNA
Messenger RNA; combo of RNA molecule and ribosome, directs protein synthesis