W3 - COLONY MORPHOLOGY

Question 1

Mueller Tellurite

Answer 1

Selective agent: potassium tellurite
Use: isolation of C. Diptheriae

Question 2

Enterococcus Faecalis broth

Answer 2

Selective agent: Na Azide Tetrazolium
Uses: isolation of enterococci

Question 3

Phenyethanol agar; selective agent?

Answer 3

Selective agent: phenylethanol chloride

Question 4

Uses of phenylethanol agar

Answer 4

Isolation of staph and strep

Question 5

Isolation of Lactobacilli

Answer 5

Medium: Tomato juice agar
Selective agent: tomato juice, acid

Question 6

Isolation of gram (-) enteric

Answer 6

Medium: MacConkey
Selective agent: bile, crystal violet

Question 7

Selective agent: bile, citrate, brilliant green dye

Answer 7

Medium: SSA (Salmonella Shigella Agar)
Use: isolate salmonella and shigella

Question 8

Selective agent: malachite green

Answer 8

Medium: Lowenstein-Jensen agar
Use: isolation and maintenance of mycobacteria

Question 9

Use: isolation of fungi

Answer 9

Medium: Sabouraud’s agar
Selective agent: pH 5.6